Method for screening monoclonal antibody pair not interfered by complement

A monoclonal antibody and complement technology, which is applied in the field of immune detection, can solve the problems of complement interference that cannot be solved in immune diagnosis, less use of poultry antibodies, etc., and achieve the effect of reducing the probability of interference by complement and accurate screening results

Pending Publication Date: 2022-06-07
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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Problems solved by technology

It is also recommended to use poultry such as chicken IgY antibodies to replace antibodies from mice, rabbits and other sources without complement interference. H

Method used

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  • Method for screening monoclonal antibody pair not interfered by complement
  • Method for screening monoclonal antibody pair not interfered by complement
  • Method for screening monoclonal antibody pair not interfered by complement

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Embodiment 1

[0031] In this example, the screening of anti-Mullerian hormone (AMH) monoclonal antibody pairs free from complement interference is taken as an example, and complement interference is reduced in diagnostic raw materials, making the antibody pairs more suitable for chemiluminescence immunodetection.

[0032] Therefore, the present invention provides a method for screening monoclonal antibody pairs that do not interfere with complement, comprising the following steps:

[0033] S10: obtain a negative sample mixture;

[0034] S20: Add antigen to the negative sample mixture to obtain test sample 1;

[0035] S30: heating the negative sample mixture and adding antigen to obtain test sample II;

[0036] S40: adding complement C1q to the second test sample to obtain the third test sample;

[0037]S50: using the chemiluminescence reagent prepared by the monoclonal antibody pair to be selected, perform immunochemiluminescence detection on the detection sample 1, the detection sample 2...

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Abstract

The invention provides a method for screening monoclonal antibody pairs free of complement interference. The method comprises the following steps: S10, obtaining a negative sample mixture; s20, adding an antigen into the negative sample mixture to obtain a detection sample I; s30, heating the negative sample mixture, and adding an antigen to obtain a detection sample II; s40, adding complement into the detection sample II to obtain a detection sample III; s50, respectively performing immunochemiluminescence detection on the detection sample I, the detection sample II and the detection sample III by using a chemiluminescence reagent prepared from a monoclonal antibody pair to be selected to obtain a detection result I, a detection result II and a detection result III; s60, according to the first detection result, the second detection result and the third detection result, the monoclonal antibody pair free of complement interference is obtained. The technical problem that immunodiagnosis is often interfered by complements and the accuracy of immunodetection is influenced is solved, and the technical effects that the immunodiagnosis is not easily interfered by the complements and the accuracy of the immunodiagnosis is improved are achieved.

Description

technical field [0001] The present invention relates to the technical field of immunodetection, in particular, to a method for screening monoclonal antibody pairs that are not interfered by complement. Background technique [0002] Immunodiagnosis, especially the diagnostic technology based on double antibody sandwich, has developed rapidly in recent years due to its high sensitivity and specificity, such as enzyme-linked immunosorbent assay, immunochromatography, and chemiluminescence detection technology. In particular, immunochemiluminescence detection technology has gradually become the main clinical diagnostic technology due to its advantages such as high sensitivity, fast speed, and automation. However, in clinical applications, immunodiagnosis is often affected by interfering substances in samples. [0003] The interfering substances in the samples include rheumatoid factor (RF), heterophilic antibodies, autoantibodies, complement, biotin, blood lipids, bilirubin, et...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N21/76
CPCG01N33/577G01N21/76
Inventor 何进军葛超坤章成龙贾江花瑞亚·富兰克林·沃克曼邹继华叶邦策
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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