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Additive for preserving dental pulp mesenchymal stem cells and application thereof

A technology of stem cells and additives, applied in the field of additives for cell preservation, can solve the problems of complicated operation, increased preservation cost, low cell proliferation rate, etc., and achieves the effects of high viability, easy preservation and good activity.

Pending Publication Date: 2022-06-24
河南循远生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preservation in liquid nitrogen can prolong the storage time of cells, but for cells that only need to be stored for a short time, the cryopreservation operation in liquid nitrogen is complicated and increases the cost of storage
Moreover, the cryopreservation process will significantly change the thermodynamic and physical environment of the cells, which will cause some damage to the dental pulp stem cells, and the vitality of the cells cannot be guaranteed, and the cell proliferation rate is low, which affects the clinical application of the dental pulp stem cells.

Method used

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  • Additive for preserving dental pulp mesenchymal stem cells and application thereof
  • Additive for preserving dental pulp mesenchymal stem cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] An additive for preservation of dental pulp mesenchymal stem cells, which is composed of the following parts by weight of raw materials: 4.7 parts of canola protein polypeptide with molecular weight ≤3kDa, 3.5 parts of gluten, 5.4 parts of hyaluronic acid, 7.0 parts of glucose, L -8.5 parts of glutamine, 6.8 parts of D-mannitol.

[0025] The application of the above-mentioned additives, the above-mentioned additives are added to the DMEM / F12 medium to obtain a mixed solution for preservation and cooled to 2 ℃ for subsequent use, and the weight ratio of the additive and the DMEM / F12 medium is: 1:80, the P3 obtained by subculture The dental pulp mesenchymal stem cells were added to the above mixture, and the cell density was 2 × 10 5 1 / mL, aliquoted into 2mL storage tubes, 1.5mL per tube, and stored at 2°C.

Embodiment 2

[0027] An additive for the preservation of dental pulp mesenchymal stem cells, which is composed of the following raw materials in parts by weight: 3.5 parts of goldenrod chrysanthemum protein polypeptide with molecular weight ≤3kDa, 2.3 parts of gluten, 4.2 parts of hyaluronic acid, 5.5 parts of glucose, L -7.5 parts of glutamine, 5.4 parts of D-mannitol.

[0028] The application of the above-mentioned additives, the above-mentioned additives are added to the DMEM / F12 medium to obtain a mixed solution for preservation and cooled to 4 ℃ for subsequent use, and the weight ratio of the additive and the DMEM / F12 medium is: 1:85, the P4 The dental pulp mesenchymal stem cells were added to the above mixture, and the cell density was 3 × 10 5 1 / mL, aliquoted into 2mL storage tubes, 1.5mL per tube, and stored at 4°C.

Embodiment 3

[0030] An additive for preservation of dental pulp mesenchymal stem cells, which is composed of the following parts by weight of raw materials: 5.8 parts of canola protein polypeptide whose molecular weight is less than or equal to 3kDa, 4.5 parts of gluten, 6.0 parts of hyaluronic acid, 8.5 parts of glucose, L -10.0 parts of glutamine, 7.6 parts of D-mannitol.

[0031] The application of the above-mentioned additives, the above-mentioned additives are added to the DMEM / F12 medium to obtain a mixed solution for preservation and cooled to 6 ℃ for subsequent use, and the weight ratio of the additive and the DMEM / F12 medium is: 1:90, the P5 The dental pulp mesenchymal stem cells were added to the above mixture, and the cell density was 5 × 10 5 1 / mL, aliquoted into 2mL storage tubes, 1.5mL per tube, and stored at 6°C.

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Abstract

The invention discloses an additive for preserving dental pulp mesenchymal stem cells. The additive consists of the following components: golden imperial chrysanthemum protein polypeptide, vital gluten, hyaluronic acid, glucose, L-glutamine and D-mannitol. The additive provided by the invention comprises vegetable protein gold imperial chrysanthemum protein polypeptide and vital gluten as well as hyaluronic acid, glucose, L-glutamine and D-mannitol which can maintain the stability of the dental pulp mesenchymal stem cells, and all the components have a synergistic effect, so that the preservation of the dental pulp mesenchymal stem cells at 2-6 DEG C is realized; the preserved dental pulp mesenchymal stem cells still have the characteristics of high motility rate and good activity, and have great significance in clinical popularization and application of the dental pulp mesenchymal stem cells. The invention further provides specific application of the additive, the additive and a basic culture medium are mixed and then used for preserving the dental pulp mesenchymal stem cells, and a simple, convenient and efficient method is provided for preserving the dental pulp mesenchymal stem cells.

Description

technical field [0001] The invention relates to an additive for cell preservation, in particular to an additive for preservation of dental pulp mesenchymal stem cells and its application. Background technique [0002] Dental pulp mesenchymal stem cells are a kind of mesenchymal stem cells with self-renewal and multi-directional differentiation ability existing in dental pulp tissue. Dental pulp mesenchymal stem cells have strong proliferative ability and the potential of multi-directional differentiation. In addition to the cells that can form mineralized nodules, they can also differentiate into various types of cells after the induction of different cytokines. When the dental pulp tissue is damaged, the dental pulp mesenchymal stem cells in the dental pulp tissue will proliferate, migrate to the damaged part of the tooth and differentiate into dentin cells, repair the dentin and protect the dental pulp. Therefore, the study of dental pulp stem cells brings feasibility to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0231
Inventor 张亮付强
Owner 河南循远生物科技有限公司
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