Compounds for treatment of sepsis
A compound and technology for sepsis, which is applied in the field of compounds for sepsis treatment, achieves the effect of less side effects and improved survival rate
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Embodiment 1
[0031] 1. Reagents and animal information
[0032] Reagents: Compound Ia, Dexamethasone, LPS;
[0033] Vehicle: 0.5% methylcellulose (MC), phosphate buffered saline (PBS);
[0034] Animals: C57BL / 6 mice, male, 6-8 weeks old.
[0035] 2. Preparation of sample solution
[0036] LPS solution: Dissolve an appropriate amount of LPS powder in sterile PBS to prepare a 2 mg / ml solution for intraperitoneal injection modeling.
[0037] Compound Ia solution: Weigh compound Ia, add it into 0.5% MC solution, turn on the magnetic stirrer and stir for 30-60min, and then ultrasonicate for 15min, and a white homogeneous suspension is obtained. Then, gradient dilution was performed with 0.5% MC, vortexed evenly, and a white homogeneous suspension was obtained to obtain a low-concentration compound Ia solution.
[0038] Dexamethasone solution: Prepare by dissolving dexamethasone in 0.5% MC solution.
[0039] 3. Experimental process
[0040] Based on animal body weight, on day 0, 43 animals...
Embodiment 2
[0055] tablet preparation
[0056] ①Mix
[0057] 1. Pretreatment stage: Pass the raw and auxiliary materials through a 40-mesh sieve respectively according to the prescription composition in Table 3, and take the prescription amount for later use.
[0058] 2. One mixing: Ia, lactose and 50% silicon dioxide were mixed with a hopper mixer for 10 minutes at 20 rpm.
[0059] 3. Two-mix: Add pregelatinized starch, cross-linked PVP, and remaining silicon dioxide to the premix, mix for 10min, and rotate at 20rpm.
[0060] 4. Mixing: Add the lubricant magnesium stearate to the material in the hopper mixer, start mixing for 5 minutes, and rotate at 20 rpm.
[0061] ②Tablet:
[0062] Taking into account the results of theoretical tablet weight and particle content, a shallow arc circular punch with a diameter of 8 mm was used for tablet compression, and the hardness ranged from 4 to 9 kg.
[0063] ③Coating:
[0064] 10% Opadry coating solution was prepared and coated, and the final...
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