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Molecular marker of high-yield and high-quality gene GW3p6 of hybrid rice and application of molecular marker

A molecular marker, rice technology, applied in the fields of botany and molecular biology, can solve the problem that the yield-related heterosis gene is not very clear and so on

Pending Publication Date: 2022-07-01
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in this field, the yield-related heterosis genes from the female parent are still not very clear, and further in-depth research is urgently needed

Method used

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  • Molecular marker of high-yield and high-quality gene GW3p6 of hybrid rice and application of molecular marker
  • Molecular marker of high-yield and high-quality gene GW3p6 of hybrid rice and application of molecular marker
  • Molecular marker of high-yield and high-quality gene GW3p6 of hybrid rice and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1. Designing molecular markers of GW3p6 by using parental genome differences

[0078] 1. Molecularly designed plant materials

[0079] In this example, the main material used is hybrid rice, the male parent (reincarnation parent) material is Fuhui 676 (Fuhui676 or FH676), and the female parent (donor parent) material is Guangzhan 63-4S (Guangzhan63-4S or GZ63 -4S).

[0080] The hybrid progeny of Fuhui 676 and Guangzhan 63-4S is an excellent hybrid rice variety Guangliangyou 676 (GLY-676).

[0081] In order to maintain hybrid vigor, in this example, plants that retain the excellent traits of the female parent (large grain type, high grain weight) are obtained, their sequences are analyzed, and molecular markers for such excellent traits can be selected by screening.

[0082] 2. Molecular marker design

[0083] The inventors first performed 160-fold high-coverage second-generation sequencing on the parent, using Nipponbare IRGSP 4.0 as the template sequence, an...

Embodiment 2

[0106] Example 2. Determination of allele GW3p6 by Sanger sequencing

[0107] After purifying the amplified fragment in Example 1, it can be further subjected to sequencing reaction.

[0108] 1. First-generation Sanger sequencing

[0109] Genotyping was performed by first-generation sequencing, and the inventors used a fluorescent-labeled dideoxy sequencing kit (BigDye TM ) combined with ABI3730 sequencer for next-generation sequencing.

[0110] (1) Sequencing reaction

[0111] Configure a 10μL sequencing reaction system:

[0112] BigDye 3.1premix 1μL;

[0113] 5×CSA buffer 1.5μL;

[0114] DMSO 0.3 μL;

[0115] One-way primer (5pmol / l) 1μL;

[0116] DNA X μL;

[0117] ddH 2 O make up to 10 μL;

[0118] Generally, the amount of sequencing DNA is required to exceed 200ng.

[0119] Conditions for sequencing reactions:

[0120] Pre-denaturation at 95°C for 3min;

[0121] Denaturation at 95 °C for 10 sec, annealing at 50 °C for 5 sec, extension at 60 °C for 1 min, 35 c...

Embodiment 3

[0130] Example 3. Rapid realization of improved restorer line by GW3p6 molecular marker

[0131] In order to further verify the breeding effect of the molecular marker of the present invention, the inventors used the molecular marker CS-92 to introduce the GW3p6 gene from the maternal parent into the paternal restorer line by backcrossing, and bred only a 5.9-kb maternal genotype replacement The near-isogenic line NIL-GW3p6 ( Figure 4 ).

[0132] The inventors carried out fine phenotypic statistics on the near-isogenic line and the male parent Fuhui 676, and found that the overall difference between the two plants was not large ( Figure 4 A). But specific to important agronomic traits, NIL-GW3p6 increased grain length by about 7.2%, 1000-grain weight by about 6.7%, and yield per plant by about 8.5% compared with the control parent Fuhui 676 ( Figure 4 C, E, F). Interestingly, the NIL-GW3p6 plants headed about 1–2 days later than the male parent ( Figure 4 L), while ot...

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Abstract

The invention provides a molecular marker of a high-yield and high-quality gene GW3p6 of hybrid rice and application of the molecular marker. The invention discloses a molecular marker which can be applied to breeding of rice filial generations to realize rapid improvement of rice varieties. The GW3p6 not only can increase the yield, but also improves the appearance quality of the rice. Therefore, breeding by using the molecular marker is an excellent method for rapidly cultivating high-yield and high-quality rice.

Description

technical field [0001] The invention belongs to the fields of botany and molecular biology, and more particularly, the invention relates to a molecular marker of a high-yield and high-quality gene GW3p6 in hybrid rice and its application. Background technique [0002] Heterosis is a ubiquitous biological phenomenon in the biological world, which means that after two parents with different genetic compositions are crossed, the offspring are superior to both parents in terms of growth potential, biomass, survival and reproduction ability, adaptability and stress resistance. The phenomenon. Various studies have shown that heterosis is the result of the interaction of the genomes of both parents, which affects many life processes of growth and development, and is a very complex biological phenomenon. [0003] Since the 1930s, hybrid rice technology has been used on a large scale, and then plant hybrid breeding technology has been widely used in agricultural and other production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/02A01H1/04
CPCC12Q1/6895A01H1/02A01H1/04C12Q2600/13
Inventor 韩斌王长盛黄学辉蔡云霄洪磊王子群
Owner CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
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