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Montmorillonite-melanin composite hemostatic powder as well as preparation method and application thereof

A montmorillonite and melanin technology, applied in application, powder delivery, drug combination and other directions, can solve the problems of allergic reaction limitation in vivo, increase platelet adhesion, accelerate coagulation process, etc., achieve good ion exchange, improve hemostatic performance, promote sticking effect

Active Publication Date: 2022-07-08
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The porous structure and amino groups of chitosan hemostatic material can increase the adhesion of platelets and promote the aggregation of blood components such as red blood cells to form thrombus at the wound site (Du, X., Liu, Y., Yan, H., et al .Anti-infective and pro-coagulant chitosan-based hydrogel tissue adhesive for sutureless wound closure.Biomacromolecules 2020,21,1243-1253), however, its refractory degradation and easy triggering of allergic reactions in the body limit its application
Mesoporous silica can promote the activation of coagulation factor XII, accelerate coagulation cascade reaction and promote coagulation (Wang, C., Zhou, H., Niu, H., et al. Tannic acid-loaded mesoporous silica for rapid hemostasis and antibacterial activity , Biomaterials Science.2018, 6, 3318-3331), but the potential metabolic toxicity and the difficulty of cleaning powder particles in the wound limit its application
Zeolite-based hemostatic materials can absorb moisture in the blood at the wound and promote the aggregation of platelets and coagulation factors. At the same time, the calcium ions released by it can activate platelets and accelerate the coagulation process (Liang, Y., Xu, C., LiuF., et al. al.Eliminating heat injury of zeolite in hemostasis via thermalconductivity of graphene sponge.ACS Applied Materials Interfaces.2019,11,23848-23857), however, its severe exothermic effect may cause secondary damage to the wound

Method used

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  • Montmorillonite-melanin composite hemostatic powder as well as preparation method and application thereof
  • Montmorillonite-melanin composite hemostatic powder as well as preparation method and application thereof
  • Montmorillonite-melanin composite hemostatic powder as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Take 10 g of montmorillonite ore, dissolve it in 800 mL of deionized water (mass ratio 1:80), ultrasonically stir for 8 h, and perform suction filtration. The montmorillonite obtained by suction filtration was resuspended in 100 mL of deionized water, allowed to settle naturally for 30 s, and the precipitate was discarded. Take the upper suspension, settle it naturally for 5 minutes, discard the supernatant, take the precipitated montmorillonite ore and bake it in an oven at 60°C for 12 hours to obtain montmorillonite particles with a size ranging from 800 to 1500 nm.

[0028] Take commercially purchased feathers or hair strands, wash 3 times with acetone, 3 times with deionized water, and chop. The obtained feathers or hair strands were added to 40 mL of phosphate buffer (pH=6.8), 0.4 g of dithiothreitol (DTT) was added, and the mixture was uniformly mixed. 2 Under the atmosphere, shake at 37 °C for 12 h. Add 1 mL of 20 mg / mL proteinase K and 0.2 g of DTT, and contin...

Embodiment 2

[0031]Take 20 g of montmorillonite ore, dissolve it in 800 mL of deionized water (mass ratio 2:80), ultrasonically stir for 8 h, and perform suction filtration. The montmorillonite obtained by suction filtration was resuspended in 200 mL of deionized water, allowed to settle naturally for 30 s, and the precipitate was discarded. Take the upper suspension, settle it naturally for 5 minutes, discard the supernatant, take the precipitated montmorillonite ore and bake it in an oven at 80°C for 6 hours to obtain montmorillonite particles with a size ranging from 800 to 1500 nm.

[0032] Take commercially purchased feathers or hair strands, wash 3 times with acetone, 3 times with deionized water, and chop. The obtained feathers or hair strands were added to 40 mL of phosphate buffer (pH=6.8), 0.4 g of dithiothreitol (DTT) was added, and the mixture was uniformly mixed. 2 Under the atmosphere, shake at 37 °C for 12 h. Add 1 mL of 20 mg / mL proteinase K and 0.2 g of DTT, and continue...

Embodiment 3

[0035] Take 30 g of montmorillonite ore, dissolve it in 800 mL of deionized water (mass ratio 3:80), ultrasonically stir for 8 h, and perform suction filtration. The montmorillonite obtained by suction filtration was resuspended in 300 mL of deionized water, allowed to settle naturally for 30 s, and the precipitate was discarded. Take the upper suspension, settle it naturally for 5 minutes, discard the supernatant, take the precipitated montmorillonite ore and bake it in an oven at 60°C for 12 hours to obtain montmorillonite particles with a size ranging from 800 to 1500 nm.

[0036] Take commercially purchased feathers or hair strands, wash 3 times with acetone, 3 times with deionized water, and chop. The obtained feathers or hair strands were added to 40 mL of phosphate buffer (pH=6.8), 0.4 g of dithiothreitol (DTT) was added, and the mixture was uniformly mixed. 2 Under the atmosphere, shake at 37 °C for 12 h. Add 1 mL of 20 mg / mL proteinase K and 0.2 g of DTT, and contin...

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Abstract

The invention discloses montmorillonite-melanin composite haemostatic powder and a preparation method and application thereof.The montmorillonite-melanin composite haemostatic powder is formed by uniformly dispersing and coating natural melanin particles on the surfaces of montmorillonite particles, and surface groups and charges of the montmorillonite particles and the natural melanin particles can be in contact with each other, so that the surface of the montmorillonite particles and the natural melanin particles can be in contact with each other; platelet activation and blood coagulation cascade reaction activation are realized, so that the hemostasis process is accelerated, and the hemostasis performance of the composite material is synergistically and remarkably improved. The montmorillonite-melanin composite hemostatic powder disclosed by the invention is simple in preparation process, the montmorillonite particles and the natural melanin particles have synergistic efficient hemostatic performance, and meanwhile, the montmorillonite-melanin composite hemostatic powder is endowed with good biological safety due to low-toxicity components of the montmorillonite-melanin composite hemostatic powder.

Description

technical field [0001] The invention belongs to the technical field of functional hemostatic materials, and in particular relates to a montmorillonite-melanin composite hemostatic powder and a preparation method and application thereof. Background technique [0002] The development and utilization of safe and efficient hemostatic materials is the key to the success of pre-hospital emergency care. At present, the main hemostatic components of common hemostatic materials are chitosan, silica, zeolite, etc. The porous structure and amino groups of chitosan hemostatic material can increase the adhesion of platelets and promote the aggregation of blood components such as red blood cells to form thrombus at the wound site (Du, X., Liu, Y., Yan, H., et al. .Anti-infective and pro-coagulant chitosan-based hydrogel tissueadhesive for sutureless wound closure. Biomacromolecules 2020, 21, 1243-1253), however, its refractory degradation and easy inducing allergic reactions in vivo limi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/02A61L24/04A61K33/06A61K9/14A61P7/04A61K31/785
CPCA61L24/02A61L24/046A61K33/06A61K31/785A61K9/14A61P7/04A61L2400/04A61K2300/00
Inventor 张毅黄宗旺
Owner CENT SOUTH UNIV
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