Method for analyzing action mechanism of spleen-tonifying and kidney-nourishing prescription for treating epithelial-mesenchymal transition

Abstract

The invention relates to the technical field of pharmacology, in particular to a method for analyzing an action mechanism of a spleen-tonifying and kidney-nourishing prescription for treating epithelial-mesenchymal transition. The method comprises the following steps: screening active ingredients of various traditional Chinese medicines in the spleen-tonifying and kidney-nourishing formula; screening targets corresponding to the active components and epithelial-mesenchymal transition; screening a common target of the compound and epithelial-mesenchymal transition, and constructing a protein interaction network; carrying out GO biological function and KEGG pathway enrichment analysis on the common target by utilizing R software to obtain a visual result; and constructing a chemical component-target-signal channel network to obtain a core target. A chemical component-target-signal channel network is constructed to obtain a core target, a core signal channel is analyzed through network pharmacology, then the signal channel is verified through animal experiments, a comprehensive insight is provided for an action mechanism of the spleen-tonifying kidney-tonifying prescription for resisting epithelial-mesenchymal transition, and the spleen-tonifying kidney-tonifying prescription for resisting epithelial-mesenchymal transition is obtained. And a direction is provided for further researching the spleen-tonifying and kidney-tonifying formula for relieving renal fibrosis by inhibiting epithelial-mesenchymal transition.

Description

technical field [0001] The invention relates to the technical field of pharmacology, in particular to a method for analyzing the action mechanism of Jianpi Yishen prescription for treating epithelial-mesenchymal transition. Background technique [0002] Renal fibrosis can lead to progressive loss of renal function, manifested as renal innate cell proliferation, renal interstitial fibroblast activation, and extracellular matrix deposition, and is the most common cause of renal failure. The main effector cells of renal fibrosis are activated mesangial cells, fibroblasts and myofibroblasts. However, a large proportion of interstitial myofibroblasts are actually derived from affected tubular epithelial cells in the kidney through epithelial-mesenchymal transition. Epithelial-mesenchymal transition, as one of the main mechanisms of renal fibrosis, has also been found to promote renal fibrosis through a variety of signaling pathways, ultimately leading to renal impairment and the...

AI Technical Summary

Problems solved by technology

[0004] Previous drug studies have shown that Jianpi Yishen Fang can effectively treat chronic kidney disease and renal fibrosis, and effectively inhibit epithelial-mesenchymal transition. However, the composition of Jianpi Yishen Fang is complex and the mechanism of action is not clear enough, which limits its wide clinical application

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