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Myocardial ischemia reperfusion influence factor data detection method

A technology of myocardial ischemia and influencing factors, applied in biochemical equipment and methods, microbial determination/examination, instruments, etc., can solve the problem that blood vessels are difficult to reduce patient injury, etc., to reduce reperfusion injury, reduce cell pyroptosis, Reduce the effect of necrosis

Pending Publication Date: 2022-07-15
川北医学院附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the lack of analysis of the causes of myocardial ultrastructure, energy metabolism, cardiac function and electrophysiological changes caused by myocardial ischemia-reperfusion in the prior art, and it is difficult to dredge blood vessels. To reduce the disadvantages of harm to patients, a data detection method for myocardial ischemia-reperfusion influencing factors is proposed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The first step is to obtain a series of reactions that are prone to occur in myocardial ischemia-reperfusion through experiments and data query. A series of symptoms of cardiac ischemia-reperfusion include a series of myocardial ultrastructure, energy metabolism, cardiac function and electrophysiology. Injury changes, such as heart rate imbalance after recanalization of blood vessels, etc., and the essential reason is that cell pyroptosis occurs in myocardial ischemia;

[0028] A large number of miRNAs combined with NLRP3 were screened by bioinformatics software and microRNA chip technology, and miR-20a, which was significantly low-expressed in myocardial tissue and had myocardial protective effect, was selected. Bioinformatics analysis of miR-20a was carried out to screen Finally, the transcription factor STAT5A, which is abnormally expressed in mouse myocardial tissue in the myocardial ischemia-reperfusion injury model and has a binding site with the promoter region of...

Embodiment 2

[0042] The first step is to obtain a series of reactions that are prone to occur in myocardial ischemia-reperfusion through experiments and data query. A series of symptoms of cardiac ischemia-reperfusion include a series of myocardial ultrastructure, energy metabolism, cardiac function and electrophysiology. Injury changes, such as heart rate imbalance after recanalization of blood vessels, etc., and the essential reason is that cell pyroptosis occurs in myocardial ischemia;

[0043] A large number of miRNAs combined with NLRP3 were screened by bioinformatics software and microRNA chip technology, and miR-20a, which was significantly low-expressed in myocardial tissue and had myocardial protective effect, was selected. Finally, the transcription factor STAT5A, which is abnormally expressed in mouse myocardial tissue in the myocardial ischemia-reperfusion injury model and has a binding site with the promoter region of miR-20a, was screened out. Analysis of the low expression o...

Embodiment 3

[0055] The first step is to obtain a series of reactions that are prone to occur in myocardial ischemia-reperfusion through experiments and data query. A series of symptoms of cardiac ischemia-reperfusion include a series of myocardial ultrastructure, energy metabolism, cardiac function and electrophysiology. Injury changes, heart rate imbalance after vascular recanalization, etc., the essential cause is cell pyroptosis in myocardial ischemia;

[0056] A large number of miRNAs combined with NLRP3 were screened by bioinformatics software and microRNA chip technology, and miR-20a, which was significantly low-expressed in myocardial tissue and had myocardial protective effect, was selected. Finally, the transcription factor STAT5A, which is abnormally expressed in mouse myocardial tissue in the myocardial ischemia-reperfusion injury model and has a binding site with the promoter region of miR-20a, was screened out. Analysis of the low expression of STAT5A leads to the correspondi...

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Abstract

The invention discloses a myocardial ischemia reperfusion influence factor data detection method, which relates to the field of myocardial ischemia, and adopts the technical scheme that the method comprises the following steps: step 1, acquiring myocardial ischemia influence factors: acquiring a series of reactions easily occurring in myocardial ischemia reperfusion through experiments and data query, the essential reason is that pyroptosis occurs during myocardial ischemia reperfusion; step 2: establishing a mouse myocardial ischemia reperfusion model, setting a control group, an ischemia group and an experimental group, only performing surgical operation on the mouse in the control group, and not ligating coronary artery; analysis shows that the pyroptosis is reduced by injecting the MCC950 and Caspase-1 inhibitor for inhibiting the NLPR3 inflammasome within 5 minutes of myocardial ischemia, so that myocardial ischemia reperfusion injury is relieved, and myocardial necrosis caused by myocardial ischemia can be reduced by injecting the MCC950 and Caspase-1 inhibitor firstly when myocardial ischemia occurs.

Description

technical field [0001] The invention relates to the technical field of myocardial ischemia, in particular to a method for detecting data of influencing factors of myocardial ischemia-reperfusion. Background technique [0002] Myocardial ischemia-reperfusion injury has become a major problem for clinical and scientific researchers. Researchers at home and abroad have conducted extensive research on ischemia-reperfusion injury, but its specific mechanism is still unclear. Our group's previous study found that pyroptosis is one of the important causes of myocardial ischemia-reperfusion injury. [0003] The current myocardial ischemia-reperfusion methods lack the analysis of the causes of a series of damaging changes in myocardial ultrastructure, energy metabolism, cardiac function, and electrophysiology during myocardial ischemia-reperfusion. injury, thus requiring a myocardial ischemia-reperfusion approach. SUMMARY OF THE INVENTION [0004] The purpose of the present inve...

Claims

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Application Information

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IPC IPC(8): G16B40/00G16B5/00C12Q1/6883
CPCG16B40/00G16B5/00C12Q1/6883C12Q2600/178C12Q2600/158
Inventor 岳荣川胡厚祥罗瑜郑在勇王小波吕明明赵雪梅兰美德谭欣刘延旭罗豪文聪
Owner 川北医学院附属医院
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