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Method for preparing antibacterial peptide feed additive through duplex mixed fermentation

A feed additive and mixed fermentation technology, applied in the field of bioengineering manufacturing, achieves high potency, is conducive to large-scale mass production, and has good operation repeatability

Pending Publication Date: 2022-07-22
焦作市佰役安生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on mixed liquid fermentation of double-linked strains. Therefore, there is an urgent need for a double-linked mixed fermentation method for preparing antimicrobial peptide feed additives to meet market demand.

Method used

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  • Method for preparing antibacterial peptide feed additive through duplex mixed fermentation
  • Method for preparing antibacterial peptide feed additive through duplex mixed fermentation
  • Method for preparing antibacterial peptide feed additive through duplex mixed fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] like figure 1 , figure 2 As shown, a method for preparing antibacterial peptide feed additive by double mixed fermentation, comprising the following steps:

[0041] S1 Preparation of Bacillus licheniformis double lactate Pediococcus lactis seed liquid, Bacillus licheniformis and Pediococcus lactis are activated and inoculated into the seed liquid liquid medium for fermentation to obtain Bacillus licheniformis double lactic acid Pediococcus seed liquid; wherein Bacillus licheniformis cells The concentration is 1.8×10 8 CFU / ml, the cell concentration of Pediococcus lactis is 2.3×10 6 CFU / ml, fermentation conditions: pH value 6.5, fermentation temperature 30°C, fermentation time 36h.

[0042] Bacillus licheniformis is preserved in the General Microbiology Center of China Microorganism Culture Collection Management Committee, the preservation time is December 29, 2021, and the preservation number is CGMCC No. 24209; the source of the Pediococcus lactis is CICC, and the ...

Embodiment 2

[0050] A method for preparing antibacterial peptide feed additive by double mixed fermentation, comprising the following steps:

[0051] S1 Preparation of Bacillus licheniformis double lactate Pediococcus lactis seed liquid, Bacillus licheniformis and Pediococcus lactis are activated and inoculated into the seed liquid liquid medium for fermentation to obtain Bacillus licheniformis double lactic acid Pediococcus seed liquid; wherein Bacillus licheniformis cells The concentration is 2.5×10 9 CFU / ml, the cell concentration of Pediococcus lactis is 3.5×10 7 CFU / ml, the fermentation conditions are: pH value is 5.5, fermentation temperature is 25°C, and fermentation time is 48h.

[0052] Bacillus licheniformis is preserved in the General Microbiology Center of China Microorganism Culture Collection Management Committee, the preservation time is December 29, 2021, and the preservation number is CGMCC No. 24209; the source of the Pediococcus lactis is CICC, and the strain number is ...

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Abstract

The invention belongs to the field of bioengineering manufacturing, and discloses a method for preparing an antibacterial peptide feed additive through duplex mixed fermentation, which comprises the steps of bacillus licheniformis duplex pediococcus acidilactici seed liquid preparation, bacillus licheniformis duplex pediococcus acidilactici fermentation liquid preparation, high-pressure homogenate wall breaking, separation, ultrafiltration purification, spray drying and the like. The specific bacillus licheniformis (CGMCC No.24209) and pediococcus acidilactici are selected for duplex mixed fermentation, so that the antibacterial peptide crude product obtained after duplex mixed fermentation is high in antibacterial titer and has a very good antibacterial effect on wheat gibberella; the characteristic that aerobic bacteria and anaerobic bacteria are complementary in physiological characteristics in the fermentation process of bacillus licheniformis and pediococcus acidilactici is utilized, so that the activity of an antibacterial peptide crude product obtained after fermentation is higher.

Description

technical field [0001] The invention relates to the field of bioengineering manufacturing, in particular to a method for preparing an antimicrobial peptide feed additive by double mixed fermentation. Background technique [0002] The use of traditional antibiotics and fungicides has led to the emergence of resistance in more and more pathogenic microorganisms and environmental pests, such as allergic reactions to β-lactam antibiotics and the emergence of drug-resistant strains due to long-term use. [0003] In the breeding industry, due to the long-term use of antibiotics in the breeding process, the pathogenic microorganisms have developed drug resistance, resulting in environmental pollution, threatening food safety and other prominent problems. Therefore, antibiotic alternatives have become a hot spot in market demand. Antibacterial peptides have the advantages of no residue, no drug resistance, and promote animal intestinal health. [0004] Antibacterial peptides, as a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/147A23K10/16A23L3/3526C12P21/00C12P39/00C07K1/34C12R1/10C12R1/01
CPCA23K20/147A23K10/16A23L3/3526C12P21/00C12P39/00C07K1/34A23V2002/00A23V2200/10A23V2250/55
Inventor 常艺海王文龙牛俊轲李高强袁利芹刘永立张杰段丹霞练豪帅刘祖琳
Owner 焦作市佰役安生物工程有限公司
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