Rapid liquid changing device for cell culture pore plate

Abstract

The invention discloses a quick liquid changing device for a cell culture pore plate, which comprises a bracket, a plurality of liquid changing hoppers are arranged below the bracket, the liquid changing hoppers are in one-to-one correspondence with the positions of culture pores in the cell culture pore plate, and the diameter of the upper end of each liquid changing hopper is larger than that of the lower end; after the liquid changing hopper is inserted into the culture hole, an accommodating space for accommodating culture cells is reserved between the outer wall of the liquid changing hopper and the inner wall of the culture hole; after the liquid changing hopper is inserted into the culture hole, the waste culture solution can be extruded to rise and overflow into the liquid changing hopper, so that the suction operation is completed. Compared with the prior art, the device has the following advantages: when a nutrient solution is replaced, the solution changing hopper is directly and slowly pressed into the culture holes through the bracket, so that the waste nutrient solution can be sucked without controlling the force, and meanwhile, the effect of simultaneously sucking a plurality of culture holes is also realized, so that experimenters can conveniently replace the nutrient solution in the culture holes in batches; a large amount of time and energy of workers are saved, and the experiment efficiency is greatly improved.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a quick liquid changer for cell culture orifice plates. Background technique [0002] Cell culture plate is a kind of biological tool, which has the advantages of good transparency, non-toxicity and sterility. It is mainly used for culturing cells in biological experiments to promote cell growth and reproduction. The cell culture plate can be divided into flat bottom and round bottom according to the shape of the bottom. According to the difference of cultured cells, different cell culture plates should be selected. [0003] In the process of cell culture, it is generally necessary to replace the nutrient solution many times, so the exchange of the cell solution during the experiment is a common operation in cell biology. The waste nutrient solution generally floats on the upper end of the culture hole, and in the prior art, the operator generally sucks the waste nutrient s...

AI Technical Summary

Problems solved by technology

However, the above-mentioned operation process has the following problems: on the one hand, it is necessary to control the force when absorbing the waste nutrient solution. If the force is too high, the cultured cells in the culture well may be sucked away, and the operation cannot be completed quickly; The plate generally has more culture holes, and the large hole plate has dozens of culture holes. It will take a lot of time and energy for the staff to absorb the waste nutrient solution one by one, which will affect the efficiency of the experiment.

However, the above-mentioned technical solutions cannot solve the problem of how to control the suction strength

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