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Preparation method of non-squamous epithelial cell simulant

A technology of squamous epithelium and mimics, which is applied in the field of analysis and detection, and can solve the problems of poor test stability of non-squamous epithelial cells

Pending Publication Date: 2022-07-26
URIT MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of non-squamous epithelial cell mimics, aiming to solve the problem of poor stability of non-squamous epithelial cell testing in existing quality control materials

Method used

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  • Preparation method of non-squamous epithelial cell simulant
  • Preparation method of non-squamous epithelial cell simulant
  • Preparation method of non-squamous epithelial cell simulant

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preparation example Construction

[0032] see Figure 1 to Figure 11 , the present invention provides a method for preparing a non-squamous epithelial cell simulant, comprising the following steps: .

[0033] S1 obtains animal blood to prepare red blood cells;

[0034] Specifically, the blood of the animal is selected from the blood of amphibians, such as frogs and turtles.

[0035] Specific way:

[0036] S11 obtains amphibian blood, and centrifuges the blood for stratification to obtain a stratified liquid;

[0037] S12 removes the supernatant and the white blood cell layer of the layered liquid to obtain a red blood cell layer;

[0038] In S13, the red blood cell layer was washed by centrifugation with normal saline to obtain the red blood cells.

[0039] S2 put the red blood cells into a fixation buffer containing a fixative for solidification to obtain semi-solidified cells, and centrifuge the semi-solidified cells to obtain a precipitate;

[0040] Specifically, the fixative is an aldehyde fixative sel...

Embodiment 1

[0050] S001 filter fresh anticoagulated bullfrog blood;

[0051] S002 Centrifuge the anticoagulated bullfrog blood at 1500 r / min for 5 min, remove the supernatant and white blood cells, and obtain pure red blood cells;

[0052] S003 wash the red blood cells by centrifugation with normal saline;

[0053] S004 takes the precipitate in step 3, resuspends the cells with fixation buffer containing 0.1% formaldehyde, and solidifies at room temperature for 24h;

[0054] S005 Centrifuge the semi-solidified cells at 1500 r / min for 5 min, and discard the supernatant;

[0055] S006 was added to the fixation buffer containing 0.1% formaldehyde with nuclear highlighting reagent containing 1% acetic acid, the cells were resuspended with this solution, and the cells were solidified at 25°C for 24h;

[0056] S007 was washed with PBS buffer to remove fixative.

[0057] The prepared simulant was tested in a urine visible component analyzer based on the principle of flow microscopy, and the res...

Embodiment 2

[0059] S011 filter fresh anticoagulated bullfrog blood;

[0060] S012 Centrifuge the anticoagulated bullfrog blood at 1500 r / min for 5 min, remove the supernatant and white blood cells, and obtain pure red blood cells;

[0061] S013 wash the red blood cells by centrifugation with normal saline;

[0062] S014 takes the precipitate in step 3, resuspends the cells with fixation buffer containing 0.02% glutaraldehyde, and solidifies at room temperature for 24h;

[0063] S015 Centrifuge the semi-solidified cells at 1500 r / min for 5 min, and discard the supernatant.

[0064] S016 was added with 1% acetic acid-containing nucleophore reagent in fixation buffer containing 0.02% glutaraldehyde, and the cells were resuspended with this solution, and solidified at 25°C for 24h;

[0065] S017 was washed with PBS buffer to remove fixative.

[0066] The prepared simulant was tested in a urine visible component analyzer based on the principle of flow microscopy, and the results were as fol...

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Abstract

The invention relates to the technical field of analysis and detection, in particular to a preparation method of a non-squamous epithelial cell simulant. Obtaining animal blood to prepare red blood cells; putting the red blood cells into a fixing buffer solution containing a fixing agent for curing to obtain semi-cured cells, and centrifuging the semi-cured cells to obtain a precipitate; the method comprises the following steps: taking erythrocytes of an amphibian as a substrate, adding a fixing agent into the erythrocytes of the amphibian to obtain a precipitate, pre-treating the precipitate by using a nuclear prominence reagent at 25 DEG C to obtain a secondary precipitate, and washing the secondary precipitate to remove the fixing agent to obtain the epithelial cell simulant. The non-squamous epithelial cells can be accurately simulated after the cells are treated by preferably selecting the concentrations of the fixing agent and the nuclear prominence reagent, are obviously distinguished from other types of cells, and can be used for simulating the non-squamous epithelial cells, so that accurate quality control is carried out on analysis and determination of the non-squamous epithelial cells.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a preparation method of a non-squamous epithelial cell mimic. Background technique [0002] In the existing field of urine visible component analysis and detection, the quality control of instruments and reagents used is an important way to ensure the accuracy and reliability of the detection results. Among them, the quality control of quality control substances for urine visible component analyzers is particularly important. important. [0003] At present, the quality control materials of the urine formed component analysis fixatives on the market are divided into negative quality control materials and single quality control solutions of positive quality control materials according to their product components. Cells and other formed components. Epithelial cells are closely related to kidney health in the human body, especially non-squamous epithelial cells have ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N33/50
CPCG01N1/28G01N33/50G01N33/5005
Inventor 周永杨祝毅江丽萍
Owner URIT MEDICAL ELECTRONICS CO LTD
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