Rapid propagation method for adult chestnut plant improved variety
A plant seed and plant technology, which is applied in the field of rapid propagation of mature chestnut varieties, can solve problems such as difficulty in cultivating roots, low reproduction efficiency, and slow promotion of improved varieties in the chestnut industry.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0087] The cultivation of embodiment 1 scion
[0088] The cultivation process of scion is as figure 1 shown, as follows:
[0089] (1) Acquisition of explants: The 20-year-old national-examined chestnut variety 'Yanshan Zaofeng', a 20-year-old Chinese chestnut cultivar with strong growth, plump stems and buds, was used as the test material. The stem segments of the newly drawn unlignified young vegetative branches of the current year were collected as explants (stem segments for short). In the growing season, the best period for the first-generation culture explants of chestnut mature stems is from early May to early June, that is, the vigorous growth period of new shoots to the semi-lignified stage of new shoots. During this period, the branches are not lignified, and the metabolism is vigorous and Sufficient nutrition, suitable for chestnut tissue culture explants.
[0090] It can be seen from Table 1 that the germination rate is jointly affected by the pollution rate and ...
Embodiment 2
[0135] The cultivation of embodiment 2 rootstock
[0136] The cultivation process of rootstock is as figure 2 shown, as follows:
[0137] (1) The seeds of the national-examined chestnut variety ‘Yanshan Zaofeng’ in Qianxi County, Hebei Province were selected as the test materials, which were full, consistent in size, and free of diseases and insect pests.
[0138] (2) First wash the intact chestnut seeds with clean water 3 times, then wash with detergent water once, and use a toothbrush to rinse the external soil, fungi, etc., and then rinse them with running water for 30 minutes. Then in the ultra-clean workbench, for the first disinfection and sterilization treatment, disinfect with 75% alcohol for 30s, rinse with sterile water for 3 times, then disinfect with 5% NaClO solution for 20 minutes, rinse with sterile water for 3 times, and put the sterilized Put the seeds in a tray, pour the sterilized 1g / L PVP solution into the tray, peel the seeds with a sterilized scalpel a...
Embodiment 3
[0149] Example 3 Microbody grafting
[0150] Micrografting process such as image 3 shown, as follows:
[0151] When the embryo square tissue culture seedling reaches 3cm, it can be used as the rootstock, and the 3-4cm long mature 'Yanshan Zaofeng' tissue culture microbranch is used as the scion for micrografting.
[0152] The method is as follows: at the lower end of the scion 1-1.5cm, cut both sides straight into two horse ear-shaped surfaces, so that the scion is in the shape of a wooden wedge. Split the rootstock from the middle, insert the scion, pay attention to the cambium on one side and the two sides, align from top to bottom, the scion wound exposes the interface 0.5cm, and the wound is tightly wrapped with a dressing material parafilm.
PUM
| Property | Measurement | Unit |
|---|---|---|
| Concentration | aaaaa | aaaaa |
| Concentration | aaaaa | aaaaa |
| Length | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More