The invention discloses a preparation method of compound organic fertilizer for grapes. The preparation method comprises the following steps: preparing a soil modifier, preparing an organic fertilizer, preparing a compound organic fertilizer, and pelletizing. The compound organic fertilizer for grapes comprises the following components: cattle and sheep manure, chicken manure, urea, potassium chloride, superphosphate, zinc sulfate, vinegar residue, a soil conditioner, manioc waste, active peptide, potassium sulfate, ammonium chloride, sodium selenite, zinc sulfate, an EM bacteria agent, and a film coating agent. By adopting the preparation method, the nutritional ingredients of the fertilizer can be improved through the organic fertilizer, the quality and yield of grapes can be increased, and grapes are large and full; the nutritional ingredient content of a fermentation product of the organic fertilizer can be increased by adding active polypeptide, the fermentation time can also be shortened, the production period of the fertilizer can be shortened, and economic benefits of enterprises are increased; the preparation method of the compound organic fertilizer is simple, the raw materials are easily available, the preparation time is short, the batch production can be conducted, and the economic benefits are high.