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3D printing ink for cornea regeneration as well as preparation method and application of 3D printing ink

A 3D printing and cornea technology, applied in medical science, prosthesis, additive processing, etc., can solve the problems of high ink viscosity, decreased cell viability during printing, and cell shear force damage, etc., to achieve strong practicability and preparation The effect of simple method and wide source of materials

Active Publication Date: 2022-07-29
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past few years, digital light processing technology (DLP) has been favored by more and more researchers as a light-assisted 3D printing technology, which can solve some shortcomings in inkjet printing and extrusion printing. , such as the cells are easily damaged by shear force during the extrusion process, the high requirements for ink viscosity, the decrease of cell viability caused by too long printing time, etc.

Method used

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  • 3D printing ink for cornea regeneration as well as preparation method and application of 3D printing ink
  • 3D printing ink for cornea regeneration as well as preparation method and application of 3D printing ink
  • 3D printing ink for cornea regeneration as well as preparation method and application of 3D printing ink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of 3D printing ink and printing product (PEGDA-GelMA): use PBS to prepare a certain concentration of PEGDA solution (10wt%) and GelMA solution (5wt%) and filter bacteria through a 0.22μm filter, add a certain concentration to it at 37°C. Amount of sterile photoinitiator phenyl (2,4,6-trimethylbenzoyl) phosphate lithium salt (LAP, 0.25wt%) and light blocker tartrazine (UV absorber, 0.05wt%), in DLP The printing parameters are set in the 3D printer, the exposure time is 20s, the layer height is 50μm, and the printing temperature is 37℃.

Embodiment 2

[0036] Preparation of 3D printing ink and printing product (PEGDA-GelMA): use PBS to prepare a certain concentration of PEGDA solution (15wt%) and GelMA solution (5wt%) and filter bacteria through a 0.22μm filter, add a certain amount of bacteria to it at 37°C. Amount of sterile photoinitiator phenyl (2,4,6-trimethylbenzoyl) phosphate lithium salt (LAP, 0.5wt%) and light blocker tartrazine (UV absorber, 0.1wt%), in DLP The printing parameters are set in the 3D printer, the exposure time is 10s, the layer height is 20μm, and the printing temperature is 37℃.

Embodiment 3

[0038] Preparation of 3D printing ink and printing product (PEGDA-GelMA): use PBS to prepare a certain concentration of PEGDA solution (20wt%) and GelMA solution (5wt%) and filter bacteria through a 0.22 μm filter, add a certain amount of bacteria to it at 37°C. Amount of sterile photoinitiator phenyl (2,4,6-trimethylbenzoyl) phosphate lithium salt (LAP, 0.25wt%) and light blocker tartrazine (UV absorber, 0.05wt%), in DLP The printing parameters are set in the 3D printer, the exposure time is 30s, the layer height is 60μm, and the printing temperature is 37℃.

[0039] Characterization of the printed product (PEGDA-GelMA):

[0040] (1) NMR and infrared structural characterization of the synthesized PEGDA and GelMA, such as figure 1 As shown in the figure, there is an obvious double bond peak at 5.5-6.5ppm, indicating that acryloyl chloride has successfully reacted with the hydroxyl groups at both ends of PEG to form double bonds, which proves the successful synthesis of PEGDA....

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Abstract

The invention provides 3D printing ink for cornea regeneration and a preparation method and application thereof.The preparation method includes the steps that a phosphoric acid buffer solution is used for preparing a polyethylene glycol diacrylate solution and a methylacryloylated gelatin solution, and the polyethylene glycol diacrylate solution, the methylacryloylated gelatin solution and a photoinitiator phenyl (2, 4, 6-trimethyl-1, 3-pentanedione) are mixed and stirred to be uniform; the preparation method comprises the following steps: filtering bacteria from phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate and a light blocking agent lemon yellow through a 0.22 [mu] m filter membrane, and mixing a polyethylene glycol diacrylate solution, a methacrylated gelatin solution, a photoinitiator phenyl (2, 4, 6-trimethylbenzoyl) lithium phosphate and the light blocking agent lemon yellow in an environment of 37 DEG C to obtain the 3D printing ink. The preparation method is simple, wide in material source and high in practicability.

Description

technical field [0001] The present invention relates to the technical field of biomedical materials, and more particularly to a 3D printing ink for corneal regeneration and a preparation method and application thereof. Background technique [0002] The cornea is a transparent, multi-layered structure on the surface of the eyeball whose main function is to collect light onto the lens and then direct it to the retina. It consists of corneal epithelium, corneal stroma, and corneal endothelial layer, which is composed of stratified epithelial cells differentiated from limbal stem cells, which allow the infiltration of oxygen and essential nutrients, and hinder the entry of pathogens and dust Eye. The stroma constitutes 90% of the thickness of the cornea and is composed of highly orthogonally arranged collagen fibers containing corneal stromal cells in between. The innermost layer is the corneal endothelial layer, which is composed of a layer of endothelial cells and has poor r...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/52A61L27/58B33Y10/00B33Y70/00B33Y80/00
CPCA61L27/222A61L27/52A61L27/58B33Y10/00B33Y70/00B33Y80/00A61L2430/16C08L89/00Y02P10/25
Inventor 刘文广何彬彬
Owner TIANJIN UNIV