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Bacillus cereus, lettuce microbial fertilizer as well as preparation method and application of bacillus cereus and lettuce microbial fertilizer

A technology of Bacillus cereus and microbial fertilizer, which is applied in the field of Bacillus cereus, lettuce microbial fertilizer and its preparation, can solve the problems of excellent growth-promoting effect of microbial fertilizer, low safety, and low utilization rate of endophytic bacteria. High-level problems, to improve crop quality, promote growth, and solve biological security problems

Pending Publication Date: 2022-07-29
NANCHANG HANGKONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to provide a microbial fertilizer that can promote the growth and development of lettuce to reduce the use of chemical fertilizers, and the microbial fertilizer can promote the growth of endophytic bacteria. Excellent effect

Method used

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  • Bacillus cereus, lettuce microbial fertilizer as well as preparation method and application of bacillus cereus and lettuce microbial fertilizer
  • Bacillus cereus, lettuce microbial fertilizer as well as preparation method and application of bacillus cereus and lettuce microbial fertilizer
  • Bacillus cereus, lettuce microbial fertilizer as well as preparation method and application of bacillus cereus and lettuce microbial fertilizer

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preparation example Construction

[0034] The present invention also provides the preparation method of described rapeseed microbial fertilizer, comprising the following steps:

[0035] (1) Bacillus cereus is inoculated in a culture medium to make a Bacillus cereus stock solution;

[0036] (2) centrifuging the Bacillus cereus stock solution obtained in step (1) to obtain the bacterial suspension of Bacillus cereus to remove the fermentation supernatant and sediment after the thalline;

[0037] (3) drying the precipitate obtained in step (2), and grinding to obtain inactivated cells of Bacillus cereus.

[0038] In the present invention, the medium described in step (1) is LB medium;

[0039] In the present invention, the culturing time in step (1) is 8-16h; preferably 9-15h; more preferably 10-14h; more preferably 12h.

[0040] In the present invention, the culture temperature in step (1) is 35-39°C; preferably 36-38°C; more preferably 37°C.

[0041] In the present invention, the rotational speed of the centr...

Embodiment 1

[0055] A preparation method of rapeseed microbial bacterial fertilizer, the steps are as follows:

[0056] (1) Inoculate Bacillus cereus in LB medium at 35°C, and cultivate for 8 hours under the condition that the rotating speed of the constant temperature shaker is 140 rpm to prepare a Bacillus cereus stock solution;

[0057] (2) centrifuging the Bacillus cereus stock solution obtained in step (1) to obtain the bacterial suspension of Bacillus cereus to remove the fermentation supernatant and sediment after the thalline;

[0058] The rotating speed of described centrifugal is 7500rpm; The time of described centrifugal is 4min;

[0059] (3) drying the precipitate obtained in step (2) at 150° C. for 2 h, and grinding, passing the ground precipitate through a 60-mesh sieve, and collecting the fractions under the sieve to obtain inactivated cells of Bacillus cereus.

Embodiment 2

[0061] A preparation method of rapeseed microbial bacterial fertilizer, the steps are as follows:

[0062] (1) Inoculate Bacillus cereus in LB medium at 39°C, and cultivate for 16 hours under the condition that the speed of constant temperature shaker is 160 rpm to prepare Bacillus cereus stock solution;

[0063] (2) centrifuging the Bacillus cereus stock solution obtained in step (1) to obtain the bacterial suspension of Bacillus cereus to remove the fermentation supernatant and sediment after the thalline;

[0064] The rotating speed of described centrifugal is 8500rpm; The time of described centrifugal is 6min;

[0065] (3) drying the precipitate obtained in step (2) at 170° C. for 4 hours, and grinding, passing the ground precipitate through an 80-mesh sieve, and collecting the fractions under the sieve to obtain inactivated cells of Bacillus cereus.

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Abstract

The invention relates to the technical field of microbial fertilizers, in particular to a bacillus cereus strain, a lettuce microbial fertilizer and a preparation method and application of the bacillus cereus strain and the lettuce microbial fertilizer. The invention provides a strain of bacillus cereus, wherein the Latin name of the bacillus cereus is bacillus cereus; the preservation number is GDMCC No: 62282. According to the invention, a strain of bacillus cereus is separated from solanum nigrum stems, and metabolites and inactivated bacteria generated by the bacillus cereus can significantly promote the growth of the leaf lettuce and improve the quality of the leaf lettuce.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizers, in particular to a Bacillus cereus, rapeseed microbial fertilizer and a preparation method and application thereof. Background technique [0002] Lettuce (Lactuca sativa L.) is one of the most important vegetable foods, belonging to the genus Lettuce of Asteraceae. Because it contains a lot of vitamins, carotene and phenolic compounds, it has a positive effect on reducing cardiovascular diseases and chronic diseases, and it is also an important source of protein, minerals and trace elements, and is widely loved by consumers. In recent years, the extensive use of chemical pesticides and fertilizers has resulted in soil compaction and low fertility, resulting in a decline in growth potential, yield and quality of rapeseed during the growth process. [0003] Endophyte refers to the internal fungi or bacteria that live in the tissues or organs of healthy plants at a certain stage or at...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01C1/00A01G22/15A01C21/00C12R1/085
CPCC12N1/20A01C1/00A01G22/15C12N1/205A01N63/22A01P21/00C12R2001/085Y02A40/10
Inventor 肖潇肖启程万聿可冯炜然黄雨田王欣任伟任重刘婷罗旭彪罗胜联
Owner NANCHANG HANGKONG UNIVERSITY
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