Molecular marker related to degradation and self-capping of watermelon tendrils and application of molecular marker

A molecular marker, self-capping technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as unclear regulation mechanism of self-capping traits

Pending Publication Date: 2022-07-29
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the regulation mechanism of tendril degeneration and self-capping traits in watermelon is still unclear, and further gene mapping is needed to develop breeding molecular markers closely linked to this trait

Method used

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  • Molecular marker related to degradation and self-capping of watermelon tendrils and application of molecular marker
  • Molecular marker related to degradation and self-capping of watermelon tendrils and application of molecular marker
  • Molecular marker related to degradation and self-capping of watermelon tendrils and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1. Acquisition of InDel marker ID_PB3 co-isolated from watermelon tendril degeneration and self-capping gene

[0030] 1. Materials and group construction

[0031] F1 obtained by crossing TL-DF (watermelon material with degenerated tendrils and self-capping) as the female parent, with Sanbaigua (watermelon material with normal tendrils growing indefinitely) as the male parent, and using the above-mentioned TL-DF female parent and Sanbaigua as the male parent. Generation, the F1 generation backcrossed with TL-DF to obtain the BC1P1 generation population, while the F1 generation self-crossed one generation to obtain the F2 generation population.

[0032] 2. Preliminary localization of tendril degeneration and self-capping genes

[0033] In the spring of 2021, the parental TL-DF, Sanbaigua, F1 generation plants, 113 F2 individual plants, and 62 BC1 individual plants were planted in Wuhan, and the tendril degradation and self-capping traits were identified on the pa...

Embodiment 2

[0065] Example 2. Identification of watermelon tendrils and self-capping traits

[0066] In order to further verify the linkage relationship between the ID_PB3 marker in Example 1 and the watermelon flesh color gene, 50 natural populations were used for verification. Specific steps are as follows:

[0067] 1. Materials to be tested

[0068] The test materials are 50 watermelon natural resource groups, all of which are normal tendrils and infinite inflorescence growth type (see Table 4 for details); each test material in Table 4 is preserved by the melon research group of the Economic Crops Research Institute of Hubei Academy of Agricultural Sciences. Germplasm resources.

[0069] Table 4 Identification results of individual plant materials and tendrils and inflorescence growth types of natural populations composed of 50 watermelon germplasm resources

[0070] (+ / + in marking detection means tendrils degenerate and self-capping, + / - and - / - means tendrils grow normally and i...

Embodiment 3

[0083] Example 3. Molecular-assisted breeding of watermelon tendrils and inflorescence growth traits

[0084] Molecular marker-assisted breeding using the molecular marker ID_PB3 obtained in Example 1 using tendril degeneration and inflorescence self-capping watermelon material, including the following steps: DNA extraction: using tendril degeneration and self-capping watermelon as donor parents, other tendrils grow normally and indefinitely Watermelon is the recipient parent, and the substitution line and its derivative lines obtained by hybridization and multi-generation backcross are used to extract the genomic DNA of the isolated population by CTAB (Paterson et al. 1993) method at the seedling stage;

[0085] Use the molecular marker ID_PB3 to detect the genotype of the individual plants of the above population, and select the individual plants whose ID_PB3 marker is heterozygous (+ / -) at the 67th position in each generation for the next round of backcrossing; after 6-8 con...

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Abstract

The invention discloses a molecular marker related to degradation and self-capping of watermelon tendrils and application of the molecular marker. Whole genome sequencing is carried out on a watermelon tendril degeneration and self-capping material TL-DF and a normal tendril infinite growth type material three-white melon, SNP and INDEL molecular markers are developed by comparing with the sequence of a reference genome 97103v2, BC1 and F2 segregation populations are respectively constructed by hybridizing the TL-DF and the three-white melon, and the situation that tendril degeneration and self-capping characters appear together is found, so that the tendril degeneration and self-capping characters of the watermelon can be identified. And the gene is controlled by the same gene locus. The tendril degeneration and self-capping gene is preliminarily localized to a watermelon chromosome 4 through a BSA-seq method, and then the tendril degeneration and self-capping gene is finely localized by using a BC1 segregation population to develop a molecular marker IDPB3 co-segregated with the two characters. Experiments prove that the molecular marker IDPB3 disclosed by the invention is co-separated from tendril degeneration and self-capping characters in a watermelon natural population, and can be used for molecular marker-assisted breeding of watermelons.

Description

technical field [0001] The invention belongs to the technical field of watermelon molecular breeding, and in particular relates to a molecular marker related to the degeneration and self-capping of watermelon tendrils and its application. Background technique [0002] Watermelon (Citrullus lanatus) is an important commercial crop that is widely cultivated worldwide. According to the statistics of the United Nations Food and Agriculture Organization (FAOSTAT), in 2018, the cultivation area in my country reached 1.510 million hm2, accounting for 46.58% of the total global area, and the total output exceeded 60 million tons, accounting for 60.64% of the world's total output. Watermelon industry has become one of the important pillar industries of my country's agricultural economic development. [0003] Watermelon is a trailing cucurbit crop. Under normal circumstances, a lateral vine is produced in the axils of each leaf, and its inflorescence is an infinite growth type. A la...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2600/13C12Q2531/113C12Q2565/125
Inventor 易丽聪戴照义张毅王运强周伟邱益纯
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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