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Biosensor

A biosensor and sensing electrode technology, applied in the field of biosensors, can solve the problems of limited substrate concentration range, no proposed biosensor structure, etc., and achieve the effect of improving stability

Pending Publication Date: 2022-07-29
DONGWOO FINE CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using enzymes with high affinity for the substrate (such as lactate oxidase), the measurable concentration range of the substrate is limited, and no biosensor structure has been proposed that can overcome this problem.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] Example 1: Preparation of lactate sensor

[0116] A 180 μm-thick PET substrate was prepared as an insulating substrate.

[0117] The sensing electrodes were formed by screen printing a paste (DS-7406CF, manufactured by Daejoo Electronic Materials) on the substrate, the paste containing 3 parts by weight of Prussian blue based on 100 parts by weight of the carbon paste.

[0118] The reference electrode was formed by screen printing Ag / AgCl to be spaced apart from the sensing electrode by a predetermined distance.

[0119] A lactate oxidase reagent layer is formed on the sensing electrode. The lactate oxidase reagent layer was prepared as follows.

[0120] To 40 μl of lactate oxidase (manufactured by TOYOBO, 10 U / 1 μl stock solution) was added and uniformly mixed 40 μl of PBS buffer and 20 μl of 1-methoxy-5-methylphenazine methosulfate (Sigma Aldrich, 50 mM stock solution) to A composition for forming an enzyme reaction layer is prepared. 2.0 μl of the composition f...

Embodiment 2

[0123] A sensor was fabricated by the same method as in Example 1, except that the permeation control layer was formed into a three-layer structure in which the three layers were in contact with each other.

Embodiment 3

[0125] A sensor was fabricated by the same method as in Example 1, except that the permeation control layer was formed into a five-layer structure in which five layers were in contact with each other.

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Abstract

A biosensor according to an embodiment of the present invention comprises: a substrate; a working electrode disposed on the substrate; and a reference electrode disposed on the substrate to be spaced apart from the working electrode. The working electrode includes: a sensing electrode disposed on an upper surface of the substrate; the enzyme reaction layer is arranged on the upper surface of the sensing electrode; and a transport control layer disposed on an upper surface of the enzyme reaction layer and including a water-soluble polymer and a water-insoluble polymer. The transmission control layer is formed by mixing the water-soluble polymer and the water-insoluble polymer, and thus sensing performance with respect to a material to be detected can be improved.

Description

technical field [0001] The present invention relates to a biosensor. More specifically, the present invention relates to a biosensor comprising a working electrode and a reference electrode. Background technique [0002] The healthcare industry is rapidly expanding as people's life expectancy increases. In particular, the demand for portable and small biosensors that can conveniently measure various biosignals anywhere is increasing. [0003] For example, biosensors may use enzymes that react with chemicals contained in body fluids (sweat, tears, blood, etc.). When an enzyme reacts with a chemical to generate an electrical current, the electrical current can be measured to determine the concentration of the corresponding chemical. [0004] However, in enzyme-based biosensors, the measurable concentration range of chemicals varies depending on the type of enzyme. For example, when using an enzyme with a low affinity for the substrate, the reaction is saturated only when t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/26C12Q1/00G01N27/327
CPCC12Q1/005C12Q1/26G01N27/3272G01N27/3275G01N2333/90241C12Q1/003C12Q1/006C12Y113/12004C12Q1/32
Inventor 马东希李东烨
Owner DONGWOO FINE CHEM CO LTD