Rubber-reinforcing fiber, rubber product, tire cord, and tire
A rubber-reinforced, fiber-based technology that can be used in fiber processing, tire parts, textiles, and papermaking to solve problems such as operability and cord quality deterioration
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[0207] The present invention is described in more detail below with reference to Examples, but the present invention is not limited to the following Examples and may be changed as appropriate without departing from the scope of the present invention.
[0208] (1-1) Synthesis of nucleic acid encoding hydrophobic protein and construction of expression vector
[0209] A nucleic acid encoding the designed protein having the amino acid sequence set forth in SEQ ID NO:17 was synthesized. An NdeI site was added to the 5' end of the nucleic acid and an EcoRI site was added downstream of the stop codon. These five nucleic acids were cloned into a cloning vector (pUC118). Then, the nucleic acid was cleaved by restriction enzyme treatment with NdeI and EcoRI, and then recombined with the protein expression vector pET-22b(+) to obtain an expression vector.
[0210] (1-2) Expression of hydrophobic proteins
[0211] Escherichia coli BLR (DE3) was transformed with the pET22b(+) expression...
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