Emergency rapid liver detection kit based on RT-qPCR (real-time quantitative polymerase chain reaction) method

Abstract

The invention provides an emergency rapid liver detection kit based on an RT-qPCR method, the front side surface of a placement box body is provided with a placement groove, a placement seat plate is placed in the placement groove, a plurality of reagent tubes are placed on the placement seat plate, the front side surface and the rear side surface of a sealing cover plate are each fixedly provided with two insertion rods, and the insertion rods are inserted into the placement groove. The front side surface and the rear side surface of the placing box body are each provided with two inserting cylinders, and the sealing cover plate is slidably connected with the placing box body through inserting rods and the inserting cylinders. The reagent tube placing device has the following beneficial effects that through the arrangement of the placing seat plate, rubber sleeve plugs, sleeving cylinders and sponge pads, reagent tubes can be stably placed on the placing seat plate; the reagent tubes stored in the placing seat plate are fixed, the damage to the reagent tubes caused by violent shaking during moving transportation is prevented, the placing seat plate and the placing box body can be quickly connected or separated, and the reagent tubes are convenient to take or place.

Description

technical field [0001] The invention belongs to the technical field of kits, in particular to a kit for emergency rapid detection of liver based on RT-qPCR method. Background technique [0002] When detecting liver dysfunction by RT-qPCR, qPCR combined with western blotting is generally used to verify whether genes regulated by mutant NBAS-C448R screened by RNA-Seq are indeed regulated by NBAS-C448R mutation. RT-qPCR reagents need to be used in this method. RT-qPCR kits generally include a box body and a cover body. The inner cavity of the box body is provided with a reagent tank for holding reagent bottles, and detection reagent bottles are placed in the reagent tank. Most of the reagent bottles in which the reagents are placed are made of glass. During storage, transportation and movement, it is easy to collide with the bottom of the box to cause damage or have poor storage stability due to shaking. Therefore, it is urgent to use the RT-qPCR method. Liver detection kits s...

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Problems solved by technology

[0002] When detecting liver dysfunction by RT-qPCR method, qPCR method combined with Western blot method is generally used to verify whether the genes regulated by mutant NBAS-C448R screened by RNA-Seq method are indeed regulated by NBAS-C448R mutation. In this method, RT-qPCR reagents need to be used. RT-qPCR kits generally include a box body and a cover body. For example, the inner cavity of the box body is provided with a reagent tank for holding a reagent bottle, and a detection reagent bottle is placed in the reagent tank. Most of the reagent bottles for storing reagents are made of glass. When storing, transporting and moving, they are easy to collide with the bottom of the box and cause damage or have poor storage stability due to shaking. Therefore, there is an urgent need for emergency rapid This problem can be solved by detecting the liver with a kit

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