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Polysaccharide free radical degradation method

A technology of free radicals and polysaccharides, applied in the field of free radical degradation of polysaccharides, can solve the problems of loss of sulfate groups, polysaccharides not having wide applicability, and reducing partial functions of heparin, etc., to achieve reduced toxicity, good quality, cheap and easy reagents The effect

Inactive Publication Date: 2004-12-22
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 3. Salt solution is only applicable to certain polysaccharides. For example, sodium nitrite can prepare low molecular weight heparin, because the sugar amine in heparin can interact with nitrite to break the glycosidic bond, but at the same time lose the sulfate group connected to the amino group , reducing the partial function of heparin
Suitable enzymes are extremely effective in degrading polysaccharides, but the specificity of glycosidases makes enzymatic degradation of polysaccharides not widely applicable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Xylan degradation, succinic acid + H 2 O 2 +Vitamin C system

[0016] Succinic acid+H 2 O 2 +Add xylan in vitamin C system, final concentration of xylan is 10mmol / L, succinic acid is 10mmol / L, H 2 O 2 5mmol / L, vitamin C 15mmol / L, degradation at 50℃ for 20 hours. After degradation, ultrafiltration, concentration and drying, the molecular weight of the product is 3kDa-5kDa.

Embodiment 2

[0017] Example 2: Hyaluronic acid degradation, acetic acid + H 2 O 2 +Vitamin C system

[0018] Acetic acid + H 2 O 2 +Add hyaluronic acid to vitamin C system, final concentration of hyaluronic acid is 15mmol / L, acetic acid is 5mmol / L, H 2 O 2 5mmol / L, vitamin C 5mmol / L, degradation at 60℃ for 10 hours. After degradation, ultrafiltration, concentration and drying, the molecular weight of the product is 4kDa-8kDa.

Embodiment 3

[0019] Example 3: Alginic acid degrades propionic acid + H 2 O 2 +Vitamin C system

[0020] Propionic acid+H 2 O 2 +Add alginic acid to the vitamin C system, the final concentration of alginic acid is 15mmol / L, propionic acid is 15mmol / L, H 2 O 2 10mmol / L, vitamin C 5mmol / L, degradation for 24 hours at 30°C. After the degradation, ultrafiltration, concentration and drying, the molecular weight of the product is 2kDa-4kDa.

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PUM

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Abstract

A free group degradation process of polyose features that the polyose is degradated in a system composed of organic acid, H2O2 and VC at 10-80 deg.C for 2-72 hr, and the polyose comes from plant, animal or microbe, and carbon number is within 10. Its advantages are the easily available cheap reagent, gentle reaction condition, easy separation of degradated product, high quality of product and low cost.

Description

(1) Technical field [0001] The invention relates to a free radical degradation method of polysaccharides and belongs to the field of biotechnology. (2) Background technology [0002] The macromolecular structure of polysaccharides restricts its bioavailability and biological activity. The use of appropriate methods to degrade polysaccharide macromolecules is polysaccharides, especially the problem of seaweed polysaccharide development that needs to be solved urgently. At present, the degradation of polysaccharides at home and abroad generally adopts acid hydrolysis, alkaline hydrolysis, salt hydrolysis or enzymatic hydrolysis. [0003] 1 Partial acid hydrolysis, the classic HCl degradation method has been used in industrial production. Under acidic conditions, the breakage of glycosidic bonds of polysaccharides is basically random. The disadvantage is that the resulting degradation products have uneven molecular weights. [0004] 2 Partial alkaline hydrolysis. Under alkaline cond...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H1/00C07H3/00C12P19/00
Inventor 曲爱琴王海仁王琪琳李守玲张华坤
Owner SHANDONG UNIV
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