Method for prediction of preeclampsia and other diseases
A blood plasma and disease technology, applied in the direction of immunoassay, biochemical equipment and methods, microbiological measurement/testing, etc., can solve the problems of cumbersome and expensive clinical operations
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Embodiment 1
[0080] Embodiment 1-(control)
[0081] figure 1 Indicates the diagnostic underseparation that occurred by simple measurement of plasma total albumin concentrations in 11 preeclamptic women and 11 matched control women.
Embodiment 2
[0082] Embodiment 2-(control)
[0083] The same method of measuring (total) albumin as in Example 1 was carried out on the same plasma samples, except that VLDL in the plasma was removed before measuring the albumin concentration. VLDL can be precipitated from each plasma sample by mixing 100 μL of plasma with 100 μL of precipitation reagent in a centrifuge tube (acceptable ratios of sample to reagent are 1:1 or 1:5 depending on the concentration of the reagent). Mix by shaking the centrifuge tubes on a vortex mixer and centrifuge at 3,000 rpm for 10 minutes. Decant the supernatant by pipetting into a clean tube. The resulting supernatant was used to determine the concentration of albumin. result in figure 2 Indicated.
[0084] right figure 1 and 2 A comparison of the data presented shows that removal of the VLDL fraction prior to measurement of albumin improves the separation of albumin concentrations between preeclamptic and control blood samples. figure 2 Compare ...
Embodiment 3
[0085] Embodiment 3-(control)
[0086] This example illustrates the separation of NEFA concentrations in Example 1 between 11 preeclampsia patient blood samples and 11 control patient blood samples. Plasma extracted from blood samples was used to test the concentration of NEFA (without removal of VLDL). To measure the concentration of NEFA, 5 μL of plasma was pipetted into the wells of a flat bottom microtiter plate. The working solution of reagent A was added, the solution (70 μL) was mixed well, and the plate was incubated at 37° C. for 10 minutes. The working solution of Reagent B (140 μL) was then added, mixed well, and the plate was incubated at 37° C. for 10 minutes. Optical density was measured at a wavelength of 550 nm on a microtiter plate reader. The absorbance of the water blank is subtracted and the concentration of NEFA is recorded, which is proportional to the final absorbance compared to a known standard.
[0087] result in image 3 Indicated.
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