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Histidine protein-phosphatase

A technology of histidine phosphatase and phosphohistidine, which is applied to peptide/protein components, anti-enzyme immunoglobulin, hydrolase, etc., can solve the problems of undetectable and unstable hydrolysis of histidine phosphatase.

Inactive Publication Date: 2005-05-11
MERCK PATENT GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difficulty compared to others is that histidine phosphatases are not stable to hydrolysis and are not detectable in standard phosphoamino acid assays

Method used

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  • Histidine protein-phosphatase

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Embodiment Construction

[0039] (A) Separation and purification

[0040] Histidine protein phosphatase was isolated from rabbit liver.

[0041] About 110 grams of rabbit liver samples were cut into small pieces, mixed with homogenization buffer (220 ml 30 mM triethanolamine / hydrochloric acid pH 7.5, 1 mM ethylenediamine tetraacetic acid, 300 mM sucrose, 0.1 mM benzamidine, 0.1% 2-mercaptoethanol ) and grind in a homogenizer cooled on ice. After the first centrifugation (10 minutes at 3800g, 4°C), the supernatant was centrifuged again (1 hour at 48000g, 4°C). The supernatant was filtered through gauze and frozen at -80°C in approximately 20 ml aliquots.

[0042] Column chromatography separation method

[0043] The histidine protease was isolated using a three-step purification procedure (Figure 1).

[0044] 1. Anion exchange chromatography

[0045] The crude liver extract was centrifuged again (48000 g for 30 minutes). Add the supernatant to a Source Q30 (Pharmacia, Freiburg) column equilibrat...

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Abstract

The present invention relates to a novel mammalian-derived enzyme, histidine protein phosphatase, and homologue variants thereof. The invention also relates to DNA sequences encoding said proteins, methods for preparing said proteins and antibodies against them. This novel phosphatase is useful in the diagnosis of pathological states of cell regulation and cell growth, and as a drug for the treatment of pathological diseases associated with said enzyme disorders.

Description

technical field [0001] The present invention relates to a novel histidine protein phosphatase derived from mammals and its homologue variants. The invention also relates to DNA sequences encoding said proteins, methods for preparing said proteins and antibodies against them. This new phosphatase can be used in the diagnosis of pathological conditions of cell regulation and cell growth, and can also be used as a drug for the treatment of pathological diseases related to the dysfunction of said enzyme. In particular, the invention relates to the use of said enzymes in the diagnosis and treatment of diseases, the identification of agonists and antagonists of factors that play a role in histidine phosphorylation, and the potential use of agonists and antagonists in therapy. antagonist. Background technique [0002] Protein phosphorylation is a fundamental biological process that often plays a key role in signal transduction and regulation of protein ...

Claims

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Application Information

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IPC IPC(8): C12N15/55C12N9/16C07K16/40A61K38/46A61K38/00
CPCA61K38/00C12N9/16A61P25/00A61P3/00A61P35/00A61P43/00A61P9/00C07K14/435
Inventor S·克鲁普R·凯尔纳
Owner MERCK PATENT GMBH
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