Enzyme immunological method for quickly detecting rubella virus antibody

Abstract

The invention discloses an enzyme immunological method of rapidly detecting the antibody of the measles virus, mainly using PEG to speed up the antigen-antibody reaction, thus shortening the reaction time of routine ELISA. Firstly, wrap the soluble antigen in the enzyme-mark board; secondly made up the PBS lotion, PH9.6, and the sample diluent containing PEG; thirdly use the sample diluent to dilute the measured blood serum and the positive and negative comparison blood serum, fourthy add the diluted blood serum into the hole of the enzyme-mark board for warm cultivation; fifthly throw off the antigen solution in the hole, and use PBS lotion to wash; sixthly use the sample diluent to dilute the known enzyme-mark antibody; seventhly add the diluted enzyme-mark antibody in the hole for warm cultivation.

Description

technical field [0001] The invention belongs to the field of clinical medical detection, and more specifically relates to an improved enzyme immunology method for detecting rubella virus. Background technique [0002] Rubella virus is one of two viruses that can cause severe deformities in fetuses. Primary infection of rubella virus in pregnant women in the first four months of pregnancy, whether dominant or recessive, can lead to miscarriage, stillbirth and congenital rubella syndrome. Therefore, early diagnosis of rubella virus infection is extremely important for prenatal and postnatal care and improving the quality of the birth population. At present, the early diagnosis of rubella mainly depends on the detection of specific antibody in serum, that is, immunoglobulin M (IgM). Conventional indirect enzyme-linked immunosorbent assay (ELISA) is widely used in China. Due to its cumbersome operation and long process, how to simplify the operation Steps to shorten the detect...

AI Technical Summary

Problems solved by technology

[0033] Enzyme-linked capture method (EIA) is used to detect rubella virus IgM antibody, which has significantly improved specificity compared with conventional indirect ELISA method, and can eliminate the influence of RF factors that may appear in ELISA method, but this method is less sensitive than indirect ELISA method, and cumbersome steps

[0034] In addition, there is an indirect ELISA method using monoclonal antibodies as enzyme-labeled antibodies, which can also greatly improve the specificity and sensitivity of traditional methods. However, due to the complicated preparation process of monoclonal antibodies and the high price, it is not suitable for popularization.