Medicinal composition for target direction transport curing cancer and its usage
A composition and drug technology, applied in the direction of drug combination, gene therapy, non-active ingredients of polymer compounds, etc., can solve problems such as short circulation half-life
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[0046] Materials and methods
[0047] Material
[0048] L-α-phosphatidylcholine (PC), cholesterol (Chol), α1 acid glycoprotein, doxorubicin hydrochloride (Dox), N-hydroxysuccinimidyl-biotin (NHSB) and neuraminidase Purchased from Sigma Corporation (St. Louis, MO). Avidin and dipalmitoylphosphatidylserine (PS) were purchased from Calbiochem (San Diego, CA). RPMI 1640 medium, fetal bovine serum, penicillin and streptomycin were purchased from Gibco Life Technologies (Grand Island, NY).
[0049] cell culture
[0050] Human hepatoblastoma (HepG2) was obtained from the American Type Culture Collection (Rockville, MD) and maintained in RPMI 1640 medium supplemented with 5% fetal bovine serum, 100 units / ml penicillin and 100 μg / ml streptomycin at saturated humidity with 5% CO 2 cultured in an incubator. cells at 1 x 10 4 Cells / well were seeded into 96-well culture plates or 1×10 5 Cells / well were seeded into 24-well culture plates and incubated for 18-22 hours for treatme...
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