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Method for producing multiple in-phase mature ovum by diethyl stilbestrol

A technology of diethylstilbestrol and mature eggs, which is applied in the field of supernumerary synchronously mature eggs produced by diethylstilbestrol, and can solve the problem of lack of effective generation of supernumerary synchronously mature eggs

Inactive Publication Date: 2006-12-27
SHANGHAI INST OF PLANNED PARENTHOOD RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of diethylstilbestrol to produce supernumerary synchronized mature eggs
[0006] In summary, there is a lack of effective methods for producing supernumerary synchronously mature eggs in this field. Therefore, there is an urgent need to develop new methods and related reagents for effectively producing supernumerary synchronously mature eggs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Rats Produce Supernumerary Synchronously Mature Eggs Using Diethylstilbestrol

[0050] 1 Materials and methods

[0051] 1.1 Materials and reagents

[0052] 1.1.1 Experimental animals

[0053] Sprague-Dawley (S.D) rats (purchased from Bikai Experimental Animal Center, Shanghai Institute of Family Planning Sciences), New Zealand rabbits. According to the needs of the experiment, young mice of different ages were selected and used in batches.

[0054] 1.1.2 Reagents

[0055] TCM-199 medium, McCoy's-5A, fetal bovine serum (FBS), D-PBS (calcium- and magnesium-free PBS), sodium pyruvate, glutamine, non-essential amino acids, penicillin and streptomycin, etc. were purchased from GIBCO / BRL company; water, androstenedione, diethylstilbestrol, dcAMP and related culture reagents are all cell culture grade or embryo culture grade, purchased from Sigma company

[0056] 1.2 Method

[0057] 1.2.1 Preparation of undifferentiated granulosa cells and their conditioned medium

[0...

Embodiment 2

[0088] Using Diethylstilbestrol to Produce Supernumerary Simultaneously Mature Eggs in Goats

[0089] The test in Example 1 was repeated, except that goats were used instead of rats, and the dosage was changed to 1-5 mg / goat on days 1-3; 1-2.5 mg / goat on days 4-8.

[0090] As a result, a large number of supernumerary synchronously mature eggs (50-150 pieces) were also obtained.

Embodiment 3

[0092] Preparation of compositions for production of supernumerary synchronized mature eggs

[0093] Formula A (see Table 4): 10 mg of diethylstilbestrol was first dissolved with 200 microliters of 95% ethanol, then 10 ml of vegetable oil was added, and mixed to prepare a 1 mg / ml injection (the content of diethylstilbestrol was about 0.1 wt%).

[0094] formula

[0095] In addition, various injection solutions of formulations B-F in Table 4 were prepared in the same manner.

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PUM

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Abstract

The present invention provides the method of utilizing stilboestrol to produce supernumerary sync mature eggs and the composition for producing supernumerary sync mature eggs. The method of the present invention can obtain supernumerary sync mature eggs simply and effectively.

Description

technical field [0001] The invention relates to the field of biology, and more specifically relates to a method for producing supernumerary synchronous mature eggs by using diethylstilbestrol. Background technique [0002] Mammalian ovaries contain a large number of follicles in different developmental stages. Most of the follicles die in their life, and only a few follicles grow long, mature and ovulate. Therefore how to make full use of a large number of follicles in the ovary is a popular research topic for current scientific and technological workers. At present, researchers mostly choose to recover ovaries from slaughterhouses and collect oocytes in large follicles for in vitro maturation, culture, fertilization and other research. For this reason, researchers in the past devoted more energy to improving the culture system in order to increase the fertilization rate and development rate, and make full use of the limited eggs obtained, but ignored the existence and util...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/05A61P15/08
Inventor 左嘉客顾正颜桂军
Owner SHANGHAI INST OF PLANNED PARENTHOOD RES