Tooth bleaching
A technology of teeth and uses, applied in the field of oral compositions, can solve the problems of stains, insecurity, damage to teeth and/or oral tissues, etc.
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[0194] Preparation of hydroxyapatite tablets
[0195] Hydroxyapatite tablets were prepared by compressing 250 mg of hydroxyapatite in a tablet mold at a pressure of approximately 5900 kilograms (13000 Lbs) for 5 minutes. The tablets were then sintered at 600°C for 4 hours and finally hydrated with sterile deionized water.
[0196] Sterilization of Hydroxyapatite Tablets
[0197] HA tablets were sterilized at 180°C for 2 hours, hydrated with sterile deionized water, and placed in the caps of Nunc test tubes (10 ml volume).
[0198] Contamination of cattle teeth:
[0199] The bovine teeth were stained by immersing them in staining solution A at 37°C until the surface of the teeth turned brown.
[0200] Contamination of HA tablets with film
[0201] The HA tablet was immersed in 2 ml of contamination solution B placed in a Nunc test tube and rotated at an angle of approximately 30° for 5 seconds at 37° C. at 10 second intervals. Change the contaminated solution every...
Embodiment 1
[0216] Bleaching foreign tooth stains with glucose oxidase (GOX)
[0217] Bovine teeth were dipped in contamination solution A containing tea, coffee, milk and saliva at 37°C until the tooth surface turned brown, as described in the Materials and Methods section. and treated with solutions A and B and buffer as described below.
[0218] Solution A
[0219] enzyme
[0220] Two samples were prepared in Britton-Robinson buffer (pH 6.5).
[0221] Glucose solutions were prepared one day earlier and kept at 4°C.
[0222] Glucose oxidase was derived from Aspergillus niger.
[0223] Each solution was pre-incubated at 37°C for 15 minutes prior to testing.
[0224] Two teeth were placed in test tubes containing 5 ml of solution A and 5 ml of solution B, respectively, and incubated at 37°C for 60 minutes. The solution was stirred using a small magnetic bar. Both samples were treated with buffer and oxidase+glucose separately in this way. Treatments were repe...
Embodiment 2
[0231] Bleaching of extraneously contaminated hydroxyapatite tablets with L-amino acid oxidase
[0232] Exogenous contamination of hydroxyapatite (HA) tablets was carried out according to the method of Stookey (Stookey G.K. et al., "In vitro stain removal with dental cleaning water", J. Dent. Res. 61, p. 1236 (1982)).
[0233] Sterilized HA tablets (simulating the properties of human tooth enamel) as described in the Materials and Methods section (mimicking the properties of human tooth enamel) were immersed in a contamination solution containing tea, coffee, mucin, and M. luteus at 37 °C at 10-second intervals 5 seconds in B. HA tablets were incubated for 3 days. After contamination, the HA tablets were air dried and washed with deionized water.
[0234] Chromatographically pure L-amino acid oxidase from Trichoderma harzianum devoid of catalase activity was incubated with a solution of L-arginine prepared in 0.1 M sodium citrate buffer, pH 8.5.
[0235] Two of the contam...
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