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Pi-ta gene conferring disease resistance to plants

A disease-resistant gene, plant technology, applied in the direction of plant gene improvement, plant peptides, plant products, etc., can solve the problem that the nucleotide sequence of rice blast gene is not disclosed.

Inactive Publication Date: 2001-11-07
EI DU PONT DE NEMOURS & CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Likewise, the nucleotide sequence of any rice blast gene has not been published

Method used

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  • Pi-ta gene conferring disease resistance to plants
  • Pi-ta gene conferring disease resistance to plants
  • Pi-ta gene conferring disease resistance to plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0137] Example 1 Development of Pathogen Strains for Identification of Corresponding R Genes

[0138] Avirulent isolates of such pathogens are essential for the identification of host resistance genes, and the application of new pathogen strains can identify resistance genes that have hitherto evaded detection. Two fungal strains that exhibited virulence to rice varieties with specific R genes (ie, they were unable to elicit R gene-mediated defense responses) were found to lack the corresponding functional AVR genes. However, although avirulent strains of the fungus with corresponding AVR genes are necessary for the identification of host disease resistance genes, when two strains are avirulent to a particular rice variety, they may have the same AVR gene, or have R genes that trigger different Different AVR genes mediate defense responses. Application of new or unidentified fungal strains may identify previously unidentified R genes. Particular care needs to be taken when w...

Embodiment 2

[0141] Example 2 Plant Infestation and Evaluation

[0142] Infection assays were performed as previously described (Valent et al., 1991 Genetics 127:87). Conidia were collected from cultures grown on oatmeal agar plates by washing with sterile 0.25% gelatin solution. In the growth chamber, 4-5 individual plants from each rice variety were sown in Metro-Mix(R) potting medium in plastic pots. Plants were grown on a 14-hour day cycle at 28°C and a relative humidity of 70-85%. Nighttime conditions were 22°C and 85% relative humidity. Place pots with 2 week old plants in plastic bags to maintain relative humidity of 95-100% as described for pathogen infiltration, with pathogen strains such as 4360-R-62 or 4360-R as described in Example 1 -67 inoculated. With an artist's air brush (air brush) (Pasha, size 1), each ml contains 2.5 × 10 5 A 4 ml aqueous suspension of conidia was sprayed onto the plants. The plastic bags containing the inoculated plants were then twist-tie closed...

Embodiment 3

[0143] Embodiment 3 is corresponding to the identification and mapping of the R gene of rice blast AVR gene

[0144] Generate rice mapping populations. Physical markers linked to the R gene were identified using two plant populations. First, the double haploid (DH) population we used was generated at CIAT (Centro Internacional de Agriculturalura Tropical; Apdo aereo 6713, Cali, Colombia). In order to minimize the problem of segregation anomalies known to occur in crosses between japonica and indica species (Wang et al., 1994, Genetics 136:1421-1434), it was decided to combine two japonica species, Yashiro-mochi (with the R gene Pi-ta) and Tsuyuake (with the R gene Pi-km) worked. Although the level of polymorphism between the two japonica varieties is too low for the purpose of mapping the whole rice genome, considering that the Pi-ta gene originated from indica rice, there should be enough polymorphism in this R gene region. Polymorphisms enable mapping of disease-resistant...

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Abstract

The preparation and use of isolated nucleic acid fragments for conferring defense responses mediated by Pi-ta disease resistance genes against diseases caused by fungal pathogens in plants are described. The gene comprising this nucleic acid fragment and a suitable regulatory sequence alone, or the gene comprising this nucleic acid fragment and an AVR-Pita isolated nucleic acid fragment or its homologous subfragment and a suitable regulatory sequence, can be used to produce anti-multiple fungal A transgenic plant with a defense response mediated by the Pi-ta disease resistance gene of the pathogen (especially the rice blast fungus).

Description

field of invention [0001] The present invention relates to the preparation and use of an isolated nucleic acid fragment which confers a Pi-ta resistance gene-mediated defense response in plants against diseases caused by fungal pathogens. Or a gene comprising this nucleic acid fragment and suitable regulatory sequences alone, or a gene comprising this nucleic acid fragment and an AVR-Pita isolated nucleic acid fragment or a functionally equivalent subfragment thereof and suitable regulatory sequences, can be used to produce transgenic plants, the transgenic Plants can develop defense responses mediated by Pi-ta resistance genes against various fungal pathogens, especially blast fungus. Background of the invention [0002] Plants are damaged by a wide variety of disease-causing organisms including viruses, bacteria, fungi and nematodes. Invasion of a plant by a potential pathogen can have a variety of outcomes: the pathogen may succeed in multiplying in the host, causing an ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C07K14/415C12N5/10C12N15/09C12N15/29C12N15/82
CPCC12N15/8282C07K14/415
Inventor B·S·瓦伦特G·T·布赖安
Owner EI DU PONT DE NEMOURS & CO
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