Membrane transfer method for testing gene chip

Abstract

A membrane transfer method for testing gene chip includes such steps as adding digoxin or biotin label in the PCR amplification process of the specimen to be tested, hybridizing the PCR resultant with chip, binding antibody with digoxin or biotin label, and transferring the hybridized result to nylon or cellulose ucetate membrane wetted by colouring developing liquid for higher intensity of hybridized signals and higher test sensitivity.

Description

technical field [0001] The invention relates to a gene chip, in particular to a membrane transfer detection method of the gene chip. Background technique [0002] Gene chips are formed by using the intensive and parallel processing principles of microelectronic chip technology to orderly solidify a large number of biological samples with characteristic sequences of biological significance on silicon substrates or glass. Gene chips can be used to quickly obtain or process A large amount of life information (including gene identification, gene mutation detection, gene expression profile detection, etc.), which has been successfully applied in the fields of gene expression, gene mutation, gene polymorphism research and gene diagnosis. [0003] When using a gene chip, the target sample nucleic acid often needs to be amplified or cloned or reverse transcribed (mRNA) by polymerase chain reaction (PCR, hereinafter referred to as PCR), and isotope or fluorescent labeling is carried ...

AI Technical Summary

Problems solved by technology

Thus improving the current situation that chips are expensive and chip testing equipment is expensive and difficult to promote

Images