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Method of treating biological tissue for transplantation by applying ultrahigh hydrostatic pressure

一种静水压力、处理方法的技术，应用在组织再生、非有效成分的医用配制品、含有效成分的医用配制品等方向，能够解决钙化、病毒失活、很难完全地除去细胞或细菌病毒等问题

Inactive Publication Date: 2007-08-08
船本诚一
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cleaning process using only conventional surfactants or chemical solutions is insufficient to remove cellular components, bacteria, and viruses inside the tissue piece due to diffusion or infiltration of the chemical solution from the surface.
In addition, because of such limitations, it is difficult to completely remove cells or bacteria and viruses in the processing of large tissue pieces
In addition, in the case of chemical treatment, in order to achieve a sufficient effect, it is necessary to increase the degree of treatment, which will cause problems such as calcification after embedding and treatment reagents.
Moreover, the infection of BSE and CJD in dura mater transplantation has been clearly established, and it is extremely important to ensure the safety of the transplanted tissue, but the current chemical liquid treatment cannot guarantee the complete inactivation of the virus in the tissue
In addition, infection accidents from transplanted tissues often occur due to contamination during processing

Method used

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  • Method of treating biological tissue for transplantation by applying ultrahigh hydrostatic pressure
  • Method of treating biological tissue for transplantation by applying ultrahigh hydrostatic pressure
  • Method of treating biological tissue for transplantation by applying ultrahigh hydrostatic pressure

Examples

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Embodiment

[0020] 1. Purchase pig hearts from edible pig farms and transport them at 4°C. The warm ischemia time when the heart was extracted was within 20 minutes. The pulmonary valve and blood vessels were removed and cleaned with Hank's solution. The donor cells were placed in a Dr.CHEF device manufactured by Kobe Steel, and a hydrostatic pressure of 1000 to 10000 atmospheres was applied at 37°C for 10 to 30 minutes. After the treatment, it was washed and removed with PBS solution. Histological evaluation was performed by staining the tissue section of the treated specimen with HE and EVG and observing it under an optical microscope. For biomechanical properties, the heart valve leaflet after high pressure treatment is cut into a booklet with a width of 3mm and a length of about 15mm, and a tensile test is performed with a mechanical testing machine (tensilon) to measure the tension used at the time of fracture. The thickness of the valve was obtained from the measured weight and d...

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Abstract

Native tissue of mammalian origin are treated for transplantation by applying ultrahigh hydrostatic pressure to the tissue in a liquid medium. The connective tissue is rendered isolatable from the remainder of the tissue including cellular components while keeping intact the structure and biomechanical properties thereof.

Description

technical field [0001] The present invention relates to a technique for accomplishing the destruction of donor cells, cell death and virus inactivation from tissue of biological origin for transplantation. Background technique [0002] Tissue sheets for transplantation produced by chemically treating living tissue with a fixative such as glutaraldehyde or by removing cellular components in the living tissue are widely used clinically. For example, in heart valve replacement, xenogenic biological valves refer to porcine or bovine heart valves immobilized with glutaraldehyde to reduce immunogenicity. This heterogeneous biological valve has advantages in anti-coagulation, but if it is used in young people, it only has about 5 to 10 years of durability, and it is usually suitable for people over 60 years old. Europe and the United States began in 1985, and Japan has also established cryopreserved tissue banks in recent years, and cryopreserved same valves provided by cadavers a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/12A61L27/00A61K35/22A61K35/30A61K35/32A61K35/34A61K35/39A61K35/407A61K35/44A61K35/50A61L27/36A61L27/50
CPCA61K35/34A61L2430/40A61L27/3683A61K35/44A61L27/3691A61K35/12A61K47/00A61L27/00
Inventor 藤里俊哉岸田晶夫船本诚一中谷武嗣北村惣一郎
Owner 船本诚一