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Protein chip for diagnosing hepatism

A protein chip, liver disease technology, applied in the field of life science, can solve the problems of increased false positive rate, threat of experimenter, injury of detection personnel, etc., and achieves the effects of high sensitivity, safe operation and strong practicability

Inactive Publication Date: 2002-07-03
GENETECH BIOTECH SHANGHAI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The EIA kit for viral hepatitis is checked item by item, each reaction can only detect one indicator, the speed is slow, the efficiency is low, and the diagnosis is time-consuming and laborious; and the sensitivity is limited, the substrate used in the experiment is also a highly toxic chemical, which is harmful to the experiment people pose a threat
The detection of primary liver cancer by hemagglutination method and rocket method has higher sensitivity, but the false positive rate also increases; although the radioimmunoassay method is more accurate, the operation is cumbersome, and the radiation will cause harm to the detection personnel

Method used

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  • Protein chip for diagnosing hepatism
  • Protein chip for diagnosing hepatism
  • Protein chip for diagnosing hepatism

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Embodiment Construction

[0015] The liver disease diagnosis protein chip of the present invention is a high-density array of antigens and antibodies for multiple hematological detection indicators of viral hepatitis, antibodies for hematological detection indicators of primary liver cancer, positive controls, and negative controls at the same time on a solid phase carrier. , the antigen detection index and the antibody detection index are respectively located in different chambers of the microarray biochip. Antigen detection indicators include HBsAg, HbeAg, AFP); antibody detection indicators include anti-HAV IgG or IgM, HbsAb, HbeAb, HBcAb, anti-HCV IgG or IgM, anti-HDV IgG or IgM, anti-HEV IgG or IgM. The patient's serum is dripped on the surface of the chip, and the antibodies to be tested in the patient's serum bind to the corresponding antigens immobilized on the chip, and after washing away other unbound substances, add a fluorescently labeled secondary antibody to wash away the unbound substance...

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Abstract

A protein chip for diagnosing hepatism is prepared through applying the antigen and antibody relative to the viral heptitis and the antibody and positive and negativ references relative to the primary liver cancer to the solid carrier in high-density array mode to make the protein be fixed to the carrier by chemical bonds. The said protein samples are classified by antigen and antibody and applied to different chambers of microarray biochiop. Its advantages include high sensitivity, specificity and reliability.

Description

technical field [0001] The protein chip for diagnosing liver diseases of the invention belongs to the field of life sciences. A protein chip is an array of proteins or polypeptides arranged in an orderly manner on a solid substrate to achieve parallel detection, identification, identification and diagnosis of proteins. It can be applied to biology, medicine and related fields. Background technique [0002] Viral hepatitis is a statutory Class B infectious disease, mainly divided into types A, B, C, D and E; it has the characteristics of strong infectivity, complicated transmission routes, wide prevalence, high incidence rate, and difficult cure. Some patients with hepatitis B, C, and D can become chronic and develop primary hepatocellular carcinoma. Primary hepatocellular carcinoma is one of the common malignant tumors in my country, and its mortality rate ranks third in malignant tumors. It is worth noting that its incidence is on the rise, and 40% of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/576G01N33/68
Inventor 张涛李宾彭永济陈莹丁力任一萍
Owner GENETECH BIOTECH SHANGHAI