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Soybean transgene plant cultur method

A cultivation method and transgenic technology, applied in the field of biotechnology and modern agriculture, can solve the problems of low screening accuracy of transformed seedlings and slow growth of tissue culture seedlings, and achieve the effects of rooting, more pods, and rapid growth

Inactive Publication Date: 2002-11-20
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the deficiencies of the existing solid medium, provide a method for cultivating soybean transgenic plants, solve the difficulties of low screening accuracy of transformed seedlings and slow growth of tissue cultured seedlings, and achieve less chimera, fast rooting, and strong growth The effect of improving seedling pod setting rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The experimental variety is Hefeng 35

[0013] The composition of transformed shoot elongation medium is:

[0014] MSB+0.2mg / LBA+0.2mg / LIBA+0.3mg / LGA 3 +Km50mg / L,

[0015] Using solid + 0.3cm high liquid medium, cultured for 15 days, the transformed buds elongated to 3-4cm;

[0016] The composition of the transformed shoot induction rooting medium is:

[0017] 1 / 2MSB+1.5mg / LIBA+Km25mg / L,

[0018] Using solid + 0.5cm high liquid medium, culturing for 14 days to obtain soybean transgenic resistant plants;

[0019] The composition of the medium for transgenic plants is:

[0020] MSB+1.5mg / LIBA+Km25mg / L,

[0021] Use solid + 0.8cm high liquid medium, NH 4 NO 3 、KNO 3 、KH 2 PO 4 , MgSO 4 ·7H 2 O, CaCl 2 2H 2 The amount of O was reduced by 80%, flowering and pod formation were obtained, and transgenic soybean lines were obtained.

Embodiment 2

[0023] The experimental variety is Hefeng 35

[0024] The composition of transformed shoot elongation medium is:

[0025] MSB+0.2mg / LBA+0.2mg / LIBA+0.3mg / LGA 3 +Km50mg / L,

[0026] Using solid + 0.2cm high liquid medium, cultured for 18 days, the transformed buds elongated to 3-4cm;

[0027] The composition of the transformed shoot induction rooting medium is:

[0028] 1 / 2MSB+1.5mg / LIBA+Km25mg / L,

[0029] Using solid + 0.6cm high liquid medium, culturing for 18 days to obtain soybean transgenic resistant plants;

[0030] The composition of the medium for transgenic plants is:

[0031] MSB+1.5mg / LIBA+Km25mg / L,

[0032] Use solid + 0.9cm high liquid medium, NH 4 NO 3 、KNO 3 、KH 2 PO 4 , MgSO 4 ·7H 2 O, CaCl 2 2H 2 The amount of O was reduced by 70%, flowering and pod formation were obtained, and transgenic soybean lines were obtained.

Embodiment 3

[0034] The experimental variety is Hefeng 35

[0035] The composition of transformed shoot elongation medium is:

[0036] MSB+0.2mg / LBA+0.2mg / LIBA+0.3mg / LGA 3 +Km50mg / L,

[0037] Using solid + 1.0cm high liquid medium, cultured for 20 days, the transformed buds elongated to 3-4cm;

[0038] The composition of the transformed shoot induction rooting medium is:

[0039] 1 / 2MSB+1.5mg / LIBA+Km25mg / L,

[0040] Using solid + 0.7cm high liquid medium, culturing for 21 days to obtain soybean transgenic resistant plants;

[0041] Transgenic plant medium:

[0042] MSB+1.5mg / LIBA+Km25mg / L,

[0043] Use solid + 0.1cm high liquid medium, NH 4 NO 3 、KNO 3 、KH 2 PO 4 , MgSO 4 ·7H 2 O, CaCl 2 2H 2 The amount of O was reduced by 75%, flowering and pod formation were obtained, and transgenic soybean lines were obtained.

[0044] By using the method, the growth speed of the plant can be accelerated, the plant is more robust, and the biological output is increased.

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Abstract

The soybean transgenic plant culture method includes the following steps: placing advertitions bud into solid MSB bud elongating culture medium containing 50 mg / L resistomycin, at the same time adding liquid bud elongating culture medium, and placing the transformed bud into the solid MSB rooting culture medium containing 30 mg / L resistomycin, at the same time adding liquid rooting culture medium, and using liquid transformation seedling culture liquor to make culture so as to obtain the transgenic soybean seed system. Said invention adopts the combination mode of solid culture medium and liquid culture medium, and adds a certain quantity of resistomycin so as to acelerate soybean plant to root and sprout, and can inhibit growth of chimera, and can make plant quickly grow and pod more.

Description

Technical field: [0001] The invention relates to a method for cultivating soybean transgenic plants, which is a technique for biological cultivation and belongs to the fields of biotechnology and modern agricultural technology. Background technique: [0002] The exogenous target gene is introduced into recipient soybean explants through transgenic technology, and new varieties with exogenous gene characteristics are cultivated through tissue culture of transformed cells and plant regeneration, during which soybean adventitious buds are cultivated and transformed buds take root and the growth of transformed seedlings are the key links in the cultivation process. Prior art all is to adopt MSB (MS (Murashige and Skoog) inorganic component+B5 (Gamborg) organic component) solid medium (" Modern Plant Physiology Experiment Guide " Tang Zhangcheng chief editor, Science Press, 1999) in cultivation, because The contact area between the solid medium and the plant tissue is small, the...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 武天龙马晓红邱承祥
Owner SHANGHAI JIAO TONG UNIV
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