High-sensitivity homogeneous DNA hibridization fluorescence detection method

A fluorescence measurement, high-sensitivity technology, used in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of low sensitivity, and achieve the effect of high sensitivity and wide application range
CN1399126AInactive Publication Date: 2003-02-26DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2003-02-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a high-sensitivity homogeneous DNA hibridization fluorescence determination method. Eu-compound of EDTA is used to label 5'-end or 3'-end DNA probe, a kind of didentate beta-diketone is used to label 5'-end or 3'-end DNA probe for simultaneous hibridization reaction. After two labeled DNA probes are hybridized with target DNA, EDTA-Eu and beta-diketone close to each other to form ternary strong fluorescence compound EDTA-Eu-diketone. By the time identifying fluorescence process, the fluorescence strength of the formed ternary fluorescence compound is measured and the concentration of the target DNa is determined. The present invention has high sensitivty and wide application.
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Description

technical field

[0001] The invention relates to a homogeneous DNA hybridization assay technology, in particular to a high-sensitivity homogeneous DNA hybridization fluorescence assay by time-resolved fluorescence assay. Background technique

[0002] DNA hybridization assay is one of the most widely used methods for infectious disease diagnosis, gene diagnosis, tumor diagnosis, and microbial taxonomy research, as described in the literature, literature 1: S.Inoue, R.Honde, J.Clin.Microbiol., 1990, 28, 1469. Document 2: T. Sekiya, M. Fushimi, H. Hori, S. Hishmura, T. Sugimura, Proc. Natl. Acad. Sci. U.S.A., 1984, 81, 4771. Document 3: I.C. Hsu , R.A. Metcalf, T. Sun, J.A. Welsh, N.J. Wang, C.C. Harris Naure, 1991, 350, 427. However, the traditional DNA hybridization analysis method requires many steps such as stationary phase of the target DNA, pre-hybridization, hybridization, washing, etc., and the operation is complicated and time-consuming. In addition, the hybridization ...

Claims

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