Immune detection signal amplifying system and its making method and application

A signal amplification and protein technology, applied in the field of immune detection amplification system, can solve problems such as powerlessness

Inactive Publication Date: 2003-05-14
FUZHOU MAIXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even so, the SP immune signal amplification system is still powerless for the detection of very small amounts of antigens such as pathogenic microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: 1, the preparation of activated biotin:

[0042] Add 2.5mg of biotin into 30ml of dimethylformamide (DMF) solution, then add 1.5g of succinimide ester and 2.0g of diethylcarbodiimide in sequence, and stir with airtight magnetic force for 20-24 hours at room temperature. It precipitated out as a precipitate.

[0043] ●Filtrate under reduced pressure to remove the precipitate, add DMF dropwise to wash several times, and place the filtrate at 4°C overnight.

[0044] ●Filtrate under reduced pressure to remove the precipitate, heat the filtrate to about 100°C, and remove DMF under reduced pressure to obtain a solid substance.

[0045] ●Wash several times with a small amount of acetaldehyde, further remove bicycloethylcarbodiimide and solvent DMF under reduced pressure, and obtain pure activated biotin. This pure product is stored in a dry place at 4°C.

[0046] 2. Biotinylation of staphylococcal protein A (SPA)

[0047] 1ml of SPA (0.02mg / ml), adjusted to p...

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PUM

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Abstract

The present invention relates to preparation of protein, especially the immune detection and amplifying system for preparing coupling agent and its preparation and application. The present invention is one kind of immune signal amplifying system including the compound of staphylococcal protein A (SPA) and streptomycin antibiotic. The system has high detection sensitivity, short operation time.

Description

technical field [0001] The present invention relates to the preparation of protein, especially the immunodetection amplification system made by coupling agent, its preparation method and its application. Background technique [0002] Immunoassay technology is currently one of the most basic and commonly used detection technologies in the fields of medicine and biology. It is not only widely used in basic research in medical biology, but also in clinical medicine, and even in environmental monitoring. It is used in disease diagnosis and disease development. It has very important application value in the prediction of disease, the responsiveness of disease treatment, the prediction of patient prognosis, and even the monitoring of food and environmental microbial contamination. Immunological detection techniques include immunohistochemical techniques, enzyme-linked immunosorbent techniques (ELISA, etc.), immunochromatographic techniques, immunoprecipitation techniques, and immu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/532G01N33/68
Inventor 谢佐福
Owner FUZHOU MAIXIN BIOTECH CO LTD
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