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Device and method for cultivation

A culture device and culture medium technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, tissue culture, etc., can solve problems such as various culture dishes

Inactive Publication Date: 2003-12-31
HAKUJU INST FOR HEALTH SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, depending on the type of liquid substances such as cells and plasma to be cultured, or for the same test of liquid substances such as cells and plasma, the amount of the medium used varies accordingly, so there is a disadvantage that many types of culture dishes are required

Method used

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  • Device and method for cultivation
  • Device and method for cultivation
  • Device and method for cultivation

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Experimental program
Comparison scheme
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Embodiment Construction

[0012] One embodiment of the culture device of the present invention will be described in detail below with reference to the accompanying drawings. figure 1 It is summary of culture device. The culture device will be described taking the culture of cells as an example, but this culture device can also be applied to liquid substances such as plasma other than cells. The signal waveform generated by the function generator 1 is used as a power supply through the current regulator 11, which is connected in series with the culture device group 12, and the current flowing in the closed circuit is measured and represented by the ammeter.

[0013] figure 2 is a schematic diagram showing the structure of the culture device 13 . In the culture device shown in the figure, reference numeral 14 denotes a cylindrical container having a hollow inside and a specific volume. The container 14 has a concave surface 15 near the center of the bottom, and a lower electrode 16 is disposed in th...

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Abstract

A culture apparatus and cultivation method are provided which enable liquid materials such as cells or blood plasma to be evenly cultivated by making current density uniform. The culture apparatus comprises a vessel 14 having a predetermined capacity with a lower electrode 16 placed on its bottom, a cover 17 disposed in a vertically opposite direction to the vessel 14 for hermetically sealing the vessel 14 by covering the vessel 14 from above and comprising an upper electrode 18 provided opposite to the lower electrode 16 , a culturing member 20 fit into the vessel 14 comprising almost in its center a hollow 21 that has a capacity made by a predetermined cross section area; a filter 25 provided between the vessel 14 and the culturing member 20 , and a power source device for applying a predetermined voltage to the lower electrode 16 and the upper electrode 18 to let a current flow between the both electrodes.

Description

technical field [0001] The present invention relates to a culture device and a culture method for culturing liquid substances such as cells and plasma in culture medium. Background technique [0002] Figure 7 is a schematic diagram of a traditional horizontal culture device. In the figure, the current of the function generator 1 flows through the amplifier 2 and the ammeter 3 to the connecting terminals 6 of the electrodes at both ends of the culture device. The culture device applies a voltage directly on both sides of the culture tank 4, or applies a voltage between the electrodes through the agarose 5 in order to remove pollutants, so that the current flows through liquid substances such as cells and plasma in the culture solution. The value of the flowing current is obtained by the oscilloscope 7 . Figure 8 is a schematic diagram of another culture device. In this figure, too, the power source is connected to a platinum electrode 9 covered with an insulator 10, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00C12M1/42C12M3/06C12N5/07C12N5/071C12N13/00
CPCC12M35/02C12M3/00
Inventor 道解冬树伊坂胜生原昭邦
Owner HAKUJU INST FOR HEALTH SCI