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Active cell-less corium ground substance

A dermal matrix and cell technology, applied in the medical field, can solve problems such as infection, poor graft resistance to infection, and graft failure

Inactive Publication Date: 2004-01-14
RUIJIN HOSPITAL ATTACHED TO SHANGHAI NO 2 MEDICALUNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, the prominent problem encountered in the application of acellular dermal matrix is: after the transplantation of acellular dermal matrix, if a good blood circulation with the wound base cannot be established within a short period of time (3 to 5 days), severe wounds may occur. The adverse consequences of the graft are as follows: (1) the graft has poor anti-infection ability, and is prone to graft failure due to infection; (2) fails to provide sufficient nutrition for the autologous epidermis grafted on it, thereby affecting the survival of the latter

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Preparation of active acellular dermal matrix

[0066] In this example, the active acellular dermal matrix was prepared through the following steps, all of which required aseptic operation.

[0067] Step 1: Clean-grade SD rats, 24 hours after back hair removal, cut out the full-thickness skin on the back (about 15 × 20 cm2); take the medium-thick skin on the skin drum; ①Remove the epidermis: add 2.5 units / ml Dispase II (Dispase II, Roche Company), acted at 4°C for 24 hours. After the action was over, the epidermis was removed with elbow skin tweezers, and the epidermal fluid was sucked off. ② Decellularization treatment: add 160ml of 0.5% sodium dodecyl sulfate (SDS, detergent for short), and act for 24 hours at 20-26°C; absorb the detergent, rinse twice with Hanks solution, and finally, absorb Hanks solution , the acellular dermal matrix can be obtained. Then it was placed in Hanks solution containing penicillin (100u / ml), chloramphenicol (100μg / ml) and kanamycin (1...

Embodiment 2

[0071] Animal Tests of Active Acellular Dermal Matrix

[0072] In this example, animal experiments were used for testing. The test method is as follows: select a certain number of clean-grade SD rats, 24 hours after back hair removal, use a scalpel to cut a certain size of full-thickness skin on the back of the human mouse, and take the blade-thick skin on the drum-type dermatome, as an autologous Skin. The wounds were randomly divided into three groups:

[0073] The wound of group 1 was used as the experimental wound. After hemostasis, the active acellular dermal matrix prepared in Example 1 was first transplanted, and then autologous skin was transplanted on it, and bandaged under pressure;

[0074] The wound of group 2 was used as the experimental wound. After hemostasis, bFGF with a concentration of 240-24000 AU / ml or 0.1-10 ng / ml was added (volume 5-10 ml), and then acellular dermal matrix and autologous skin were successively transplanted and bandaged under pressure; ...

Embodiment 3

[0081] Preparation of active acellular dermal matrix

[0082] The active acellular dermal matrix was basically prepared according to the steps of Example 1, the only difference being that the selected skin was depilated pigskin.

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PUM

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Abstract

A non-cell active derminal matrix used for skin transplanting contains the derminal matrix containing basically no cells and the cell factor chosen from fibroblast growth factor, vascular endothelial growth factor, platelet derived growth factor, transforming growth factor-alpha, or their mixture. Its advantages are high successful rate of skin transplantation and high transplant effect.

Description

technical field [0001] The invention relates to the medical field, and more particularly relates to an active acellular dermal matrix for skin transplantation and a preparation method thereof. Background technique [0002] The modern treatment of full-thickness skin defect wounds is still based on transplanting edge-thick autologous skin (ie thin skin). Since the blade-thick autologous skin contains less dermal components, different degrees of scar hyperplasia will occur after wound healing. If thicker autologous skin is transplanted, it will often cause obvious scar hyperplasia in the donor area. [0003] In 1981, Bell et al. used extracted natural collagen to mix with fibroblasts in a certain proportion to form a dermis. Then inoculate the epidermal cell suspension on the dermis to make a composite skin similar to normal skin. [0004] Hznsbrough also used biodegradable polymers as a matrix framework for cell growth, and seeded neonatal fibroblasts in the matrix framewo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60
Inventor 陆树良向军贾生贤
Owner RUIJIN HOSPITAL ATTACHED TO SHANGHAI NO 2 MEDICALUNIV
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