Diagnostic agents for prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic kit containing same

A technology of prenatal diagnosis and diagnostic agent, applied in the direction of disease diagnosis, biological testing, biochemical equipment and methods, etc.

Inactive Publication Date: 2004-02-18
SEOUL NAT UNIV R&DB FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these substances are rarely used to predict preterm labor because their levels remain constant or are undetectable in the amniotic fluid of pregnant women with no clinical signs of preterm labor and only increase after the onset of preterm labor or premature rupture of membranes

Method used

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  • Diagnostic agents for prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic kit containing same
  • Diagnostic agents for prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic kit containing same
  • Diagnostic agents for prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic kit containing same

Examples

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Embodiment 1

[0059] The present invention can be better understood from the following examples, which are provided for illustration and should not be construed as limiting the invention. Example 1: Selection of diagnostic cut-off values ​​for prenatal diagnosis of preterm birth

[0060] There is ample evidence for the presence of chronic intrauterine infection in the etiology of preterm birth. In this Example, an experiment was performed to determine whether amniotic fluid concentrations of MMP-8 and interleukin-6 (IL-6) could be used to identify patients predisposed to spontaneous preterm labor among pregnant women without clinical signs of preterm labor.

[0061] Therefore, a case-control study was designed with preserved amniotic fluid obtained from women with genetic amniocentesis at mid-trimester. Amniotic fluid levels of MMP-8 and IL-6 were determined by ELISA in 19 patients with spontaneous preterm birth before 32 weeks' gestation and in 95 control cases of normal infants delivered...

Embodiment 2

[0064] Additionally, if figure 2 As shown, a second trimester amniotic fluid MMP-8 cut-off value of 23 ng / ml exhibited a sensitivity of 42% (8 / 19) and a specificity of 99% (94 / 95) in identifying preterm patients after genetic amniocentesis, whereas An IL-6 cutoff of 0.6 ng / ml represented a sensitivity of 42% (8 / 19) and a specificity of 92% (87 / 95). Therefore, MMP-8 is superior to IL-6 in terms of sensitivity, specificity, and predominance in identifying and predicting preterm birth. As a result, an amniotic fluid MMP-8 concentration of 23 ng / ml was selected as a cut-off value for identifying preterm birth. Example 2: Relationship between amniotic fluid concentration of MMP-8 and intrauterine infection and inflammation

[0065] Amniotic fluid concentrations of MMP-8 were examined in the presence or absence of intrauterine infection and inflammation to determine whether amniotic fluid MMP-8 concentrations could be used as a diagnostic marker for identifying intrauterine infec...

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Abstract

This invention is about a method for the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic reagent system and diagnostic kit for the diagnosis. The method, diagnostic reagent system, and kit are based on the finding that the level of MMP-8 in the amniotic fluid is significantly higher when the pregnant woman is at risk for preterm delivery, intrauterine infection, and fetal damage. The diagnostic reagent system and kit can be applied to the patients without, as well as with, clinical signs of preterm labor or premature rupture of fetal membranes. With the superiority in sensitivity and specificity as well as its less invasiveness compared to the conventional method of measuring fetal blood cytokine levels, this diagnostic reagent system and kit is very useful in the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage.

Description

field of invention [0001] The present invention relates to a diagnostic agent system for prenatal diagnosis of premature birth, fetal infection and fetal injury, and a diagnostic kit using the reagent, more particularly, relates to amniotic fluid matrix metalloproteinase-8 (MMP-8) concentration as a measure of premature birth , Application of prenatal diagnostic markers for fetal infection and fetal injury. Background of the invention [0002] It has long been recognized in the medical community that the prevention of preterm labor or premature rupture of membranes is more meaningful than its treatment after birth. However, many factors are currently known to cause preterm labor or premature rupture of membranes, making prevention of this unwanted event difficult. The traditional approach to preventing preterm birth has been to identify high-risk groups of women for whom special attention should be given based on knowledge of obstetrics and obstetrics, demographics, and var...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C12Q1/37G01N21/78G01N33/543G01N33/545G01N33/552G01N33/558G01N33/573G01N33/577G01N33/68
CPCG01N2800/36G01N2333/96486G01N33/558G01N33/54386G01N2800/368C12Q1/37G01N33/689G01N33/53
Inventor 尹保铉
Owner SEOUL NAT UNIV R&DB FOUND
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