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Preparation method of electrochemical sensor for FGFR3 (fibroblast growth factor receptor 3)-1138G>A gene polymorphism detection

A gene polymorphism, electrochemical technology, applied in scientific instruments, instruments, measuring devices, etc., can solve the problems of poor dispersion and short catalytic life, and achieve the effect of simple method, easy commercialization, and increased density of capture probes

Active Publication Date: 2017-03-08
CHONGQING MEDICAL UNIVERSITY
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Problems solved by technology

Hemin (Hemin) has attracted extensive attention due to its peroxidase-mimicking properties, but Hemin alone has poor dispersion and short catalytic life in neutral and alkaline environments, so a carrier is needed to protect Hemin

Method used

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  • Preparation method of electrochemical sensor for FGFR3 (fibroblast growth factor receptor 3)-1138G>A gene polymorphism detection
  • Preparation method of electrochemical sensor for FGFR3 (fibroblast growth factor receptor 3)-1138G>A gene polymorphism detection
  • Preparation method of electrochemical sensor for FGFR3 (fibroblast growth factor receptor 3)-1138G>A gene polymorphism detection

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Embodiment 1

[0044] Step 1. 0.126g 2-aminoterephthalic acid, 0.187g FeCl 3 ·6H 2 O and 0.226g Hemin were dissolved in 15mL DMF and mixed well. The above solution was heated in an oil bath for 4 hours, and 200 μL of glacial acetic acid was added 15 minutes after the start of heating, and the temperature of the solution was controlled to cool down to room temperature after the heating was completed. The obtained solution was centrifuged at 10000 / min for 5 minutes, and washed three times with DMF, absolute ethanol and ultrapure water respectively. The resulting product was dried under vacuum for 24 h and redispersed in ultrapure water to form 1 mg mL -1 system to obtain Hemin-MOFs;

[0045] Step 2. 1 mL H 2 PtCl 6 (1%) add 1mL (1mg mL -1 ) Hemin-MOFs solution, vigorously sonicate for 15 min. The resulting solution was placed on a magnetic stirrer, and under stirring at 400 rpm, 2 mL of NaBH was added dropwise 4 (0.1M) to the solution from brown to black, and continue to stir for 30min...

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Abstract

The invention relates to a preparation method and application of an electrochemical sensor for achondroplasia prenatal diagnosis gene-fibroblast growth factor receptor 3 (FGFR3) gene polymorphism detection, belonging to the technical field of electrochemical detection. The preparation method is characterized by comprising the following steps: synthesizing a Hemin-MOFs composite material, reducing platinum nanoparticles onto the Hemin-MOFs composite material, and mixing a single-strand DNA signal probe with the composite material to obtain a biological signal probe; and carrying out layer-by-layer self-assembly by using reducible graphene oxide tetraethylenepentamine, nano gold and avidin to fix the biotinylated DNA capture probe, thereby preparing the electrochemical sensor for FGFR3-1138G>A gene polymorphism detection. The sensor is successfully used for detecting FGFR3-gene single base mutation. The sensor has the advantages of high sensitivity and high specificity, and is quick and convenient for detection. The invention provides a new detection method for prenatal noninvasive diagnosis of achondroplasia.

Description

[0001] Technical field: [0002] The invention relates to a preparation method and application of an electrochemical sensor for prenatal non-invasive diagnosis of FGFR3-1138G>A gene polymorphism detection, especially a sandwich-type biological sensor prepared based on metal-organic framework nanocomposites as signal probes The sensor is used for detecting FGFR3-1138G>A gene polymorphism and belongs to the field of electrochemical detection. [0003] Background technique: [0004] Achondroplasia (ACH) is a common autosomal dominant genetic disease, mostly congenital dwarfism caused by defects in endochondral ossification. Although patients with this disease have normal intelligence, they have serious defects in body shape and appearance, which will cause serious psychological burden to the patients. Therefore, early prenatal genetic diagnosis of this disease is an effective measure to prevent the birth of children with achondroplasia defects. 99% of ACH patients are due t...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3278
Inventor 何俊琳于超高茹菲陈俊赵一璘
Owner CHONGQING MEDICAL UNIVERSITY
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