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Host microorganisms

A technology of microorganisms and recombinant microorganisms, applied in the field of host microorganisms, can solve problems such as insufficient improvement in productivity

Inactive Publication Date: 2004-07-14
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these strains did not show sufficient improvements in productivity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Genomic DNA was extracted from Bacillus subtilis 168. Using the extracted gene as a template, make a 1.5-kb DNA fragment (A) adjacent to the upstream end of the sigF gene (base number 2442658←2443425) on the genome, and a length adjacent to the downstream end of the sigF gene Multiplied for the 1.5-kb DNA fragment (B). Using plasmid pC194 as a template, a 0.9-kb DNA fragment containing the chloramphenicol resistance gene was individually multiplied (C). Fragments (A), (B), and (C) were sequentially ligated by SOE-PCR to prepare a 3.9-kb DNA fragment. The obtained DNA fragment was used to transform Bacillus subtilis 168 by an appropriate method. Bacillus subtilis 168 was cultured on LB agar medium containing chloramphenicol, and colonies were isolated as transformants. It was confirmed by PCR and sequencing that in the genome of the transformant, the region containing the sigF gene (2442632-2443318) was deleted and replaced by the chloramphenicol-resistant gene. Each...

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PUM

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Abstract

A microorganism wherein one or more genes selected from the group of genes participating in sporulation in the middle to late stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) by use the microorganism. No spore is formed when this microorganism is employed, thereby enabling production of a target product (a protein) while decreasing energy loss, production of a by-product and specific production speed to decrease unnecessary consumption of a medium. Moreover, the production period can be prolonged, whereby the target product (the protein) can be produced efficiently.

Description

technical field [0001] The present invention relates to host microorganisms, and recombinant microorganisms for the production of useful proteins or polypeptides. Background technique [0002] Microorganisms have been used in the industrial production of a wide range of useful substances. For example, microorganisms can be used to produce not only alcoholic beverages and foods such as miso (fermented soybean paste) and soy sauce (soy sauce), but also amino acids, organic acids, nucleic acid-related substances, antibiotics, sugars, lipids, proteins , and many other types of substances. These substances find uses in a wide range of fields, including food, pharmaceuticals, everyday essentials such as detergents and cosmetics, and as raw materials for a wide variety of chemically derived products. [0003] In industrial production of useful substances by utilizing microorganisms, an important issue is improvement of productivity. Therefore, as a measure, the cultivation of mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/32C12N1/20C12N1/21
CPCC12N1/20C07K2319/00C07K14/32
Inventor 泽田和久远藤圭二小泽忠弘东畑正敏尾崎克也
Owner KAO CORP