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An orthogonal light path type fluorescent detection device for microchip analysis

A fluorescence detection and orthogonal detection technology, which is applied in measuring devices, analyzing materials, and analyzing materials through optical means, can solve problems such as expensive, difficult to miniaturize, and complex optical system structure, and achieve low cost and reduced Effects of light scattering and high detection sensitivity

Active Publication Date: 2005-01-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The advantage of the confocal fluorescence detector is that the detection sensitivity is high, but this type of optical system has a complex structure, is expensive, and is not easy to miniaturize and integrate.

Method used

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  • An orthogonal light path type fluorescent detection device for microchip analysis
  • An orthogonal light path type fluorescent detection device for microchip analysis
  • An orthogonal light path type fluorescent detection device for microchip analysis

Examples

Experimental program
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Embodiment 1

[0024] Refer to attached figure 1 , The microchip is composed of upper 1 and lower 2 glass sheets, and the thickness of the glass sheets is 1.7 mm. A channel is processed on the upper sheet 1 by photomask and wet etching technology, and the width of the microchannel 6 is 60 microns, and the depth is 20 microns. The classic high-temperature bonding method is used to realize the permanent sealing of the upper 1 and lower 2 pieces. A microfluidic chip with a length of 60 mm and a width of 15 mm was obtained. In the present embodiment, the excitation light source 7 is a semiconductor pump laser of 473nm; the excitation light 5 emitted by the laser is focused by the condenser objective lens 4, and enters the chip microchannel 6 from a direction perpendicular to the plane of the lower chip 2 of the chip; 11 emitted, the fluorescence 8 emitted from the chip side wall 3 is collected by the collection objective lens 9 positioned behind the detection window, and the setting direction ...

Embodiment 2

[0027] Refer to attached image 3 After the excitation light 5 emitted by the light source 7 is converged by the focusing objective lens 4, it is incident from the side wall 3 of the chip in a direction perpendicular to the microchannel 6 of the chip; the fluorescent light 8 emitted from the direction perpendicular to the lower chip 2 of the chip is selected, and collected The objective lens 9 collects the stray light, and the stray light is filtered out by the optical filter, and finally the fluorescent signal is detected by the photodetector 10 .

[0028] Figure 4 It is the channel configuration diagram of the micro-analysis chip according to the preferred embodiment 1 of the present invention. The separation channel part of the microchannel 6 on the chip is designed with a bend, near the edge of the side wall 3 of the chip at the end detection area 12, in order to shorten the distance of fluorescence from the light spot to the detection window and improve the collection e...

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Abstract

The invention is an orthogonal light path type fluorescent detecting device used in microanalytical chip, composed of a light source, an optical system, and a detecting system, and its characteristic lies in that the optical system adopts an orthogonal light path, the laser emitted from the light source reaches the microanalytical chip from the side wall, and the detecting system detects the fluorescent light outgoing from the side wall. The advantages: the optical system is simple, the device is easy to make, the making cost is low, the detecting sensitivity is high, and the system is easily integrated and miniaturized.

Description

technical field [0001] The invention relates to microfluidic chip analysis, in particular to an orthogonal optical path fluorescence detection device for a microanalysis chip. Background technique [0002] The field involved in this patent is the research on the detection system on the micro-analysis chip. The detection system is an important part of an analysis system. Due to the small size of the micro-analysis chip, the injection volume is only picoliters or nanoliters, and the reaction channel at the detection site is generally tens of microns wide, so the requirements for its detection methods and devices have its own particularity. [0003] At present, one of the main detection methods used in micro-analysis chips is the fluorescence method. Among them, the laser-induced fluorescence (Laser Induced Fluorescence, LIF) detection method using a laser as the excitation light source has the characteristics of high sensitivity and easy detection on the chip. and become the...

Claims

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Application Information

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IPC IPC(8): G01N21/00H01L21/66
Inventor 方群富景林黄艳贞
Owner ZHEJIANG UNIV
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