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Method for extracting theanine from tea

A technology of theanine and tea, applied in the separation/purification of carboxylic acid amide, organic chemistry and other directions, to achieve the effect of reducing cost, not polluting the environment, and low cost

Inactive Publication Date: 2005-03-02
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the extraction of theanine from natural plants, there is no domestic reference on this aspect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take 150g of Longjing tea leaves, first soak them in 1800ml of deionized water at room temperature for 2 hours, then heat and boil for 1 hour, filter the first leaching solution, then add 1800ml of deionized water to the tea leaves, and repeat the first extraction process , and filtered to obtain the second leaching solution. The two extracts are combined to obtain the tea extract.

[0015] Adjust the pH value of the tea extract to 5.0, pass through the cation exchange resin column at a flow rate of 5.0ml / min, after loading the sample, rinse the cation exchange resin column with 500ml deionized water to remove impurities. After washing, theanine was eluted with 5% ammonia water at a flow rate of 5ml / min. Use ninhydrin chromogenic method to detect whether theanine has been eluted completely at regular intervals. After the elution is complete, 1500ml of eluate is obtained. Evaporate the eluate to dryness in a water bath at a vacuum of 0.1 MPa and a temperature of 70°C ...

Embodiment 2

[0017] Take 150g of Longjing tea leaves, soak them in 1800ml of deionized water at room temperature for 2 hours, then heat and boil for 1 hour, take the first leaching liquid, then add 1800ml of deionized water to the tea leaves, and repeat the first leaching process , take the second leaching solution. The two extracts are combined to obtain the tea extract.

[0018] Adjust the pH value of the tea extract to 5.0, and pass it through the cation exchange resin column at a flow rate of 7.0ml / min. After loading the sample, rinse the cation exchange resin column with 500ml deionized water to remove impurities. After washing, theanine was eluted with 10% ammonia water at a flow rate of 7ml / min. Use ninhydrin chromogenic method to detect whether theanine has been eluted completely at regular intervals. After the elution is complete, 600ml of eluate is obtained. Evaporate the eluate to dryness in a 70°C water bath at a vacuum of 0.1 MPa to obtain 12.1 g of theanine crude product, ...

Embodiment 3

[0020] Take 150g of old leaves of tea tree, soak them in 1800ml of deionized water at room temperature for 2 hours, then heat and boil for 1 hour, take the first leaching solution, then add 1800ml of deionized water to the tea leaves, and repeat the first leaching process , take the second leaching solution. The two extracts are combined to obtain the tea extract.

[0021] Adjust the pH value of the tea extract to 5.5, pass through the cation exchange resin column at a flow rate of 3.0ml / min, after loading the sample, rinse the cation exchange resin column with 500ml deionized water to remove impurities. After washing, theanine was eluted with 10% ammonia water at a flow rate of 3.0ml / min. Use ninhydrin chromogenic method to detect whether theanine has been eluted completely at regular intervals. After the elution was complete, 1400ml of eluent was obtained. The eluate was evaporated to dryness in a 70°C water bath at a vacuum of 0.1 MPa to obtain 13.2 g of theanine crude p...

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Abstract

The process of extracting theanine from tea includes the following steps: soaking tea with deionized water to obtain extracted solution; exchange absorbing of the extracted solution with cationic resin and elution with ammonia water to obtain theanine eluate; and decompression evaporating theanine eluate to obtain coarse theanine product. The present invention can utilize various kinds of tea material in extracting theanine, and has simple operation, low cost and no environmental pollution. The coarse theanine product is suitable for further processing and has purity of 10-30 %.

Description

technical field [0001] The invention relates to a method for extracting theanine from tea leaves. Background technique [0002] Amino acid in tea is an important component of its quality. The system is named N-ethyl-γ-L-glutamine (N-ethyl-γ-L-glatamine). Theanine is an effective flavoring substance in green tea. The content of theanine directly determines the quality of tea: no matter what kind of tea, theanine accounts for more than 50% of the free amino acids in tea, and the content of theanine in dry tea is about 1%-2%. Theanine is the ethylamine inducer of glutamic acid. It is synthesized from glutamic acid and ethylamine in the roots of tea trees, transported to the leaves for storage through the trunk, and converted into catechins under sunlight. In 1950, Mr. Shuto extracted and refined theanine from tea leaves for the first time, and determined its chemical structure (Zhao Danchao Asahi's domestic and foreign research status of theanine Food Science 2002.23 (5) 145...

Claims

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Application Information

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IPC IPC(8): C07C231/24C07C237/06
Inventor 阎卫东金鑫李胜迎
Owner ZHEJIANG UNIV
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