Method for extracting theanine from tea
A technology of theanine and tea, applied in the separation/purification of carboxylic acid amide, organic chemistry and other directions, to achieve the effect of reducing cost, not polluting the environment, and low cost
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[0013] Example 1
[0014] Take 150g of Longjing tea powder, soak it in 1800ml deionized water at room temperature for 2h, then heat and boil for 1h, filter to obtain the first extract, then add 1800ml deionized water to the tea residue, repeat the first extraction process , Filter to get the second extract. The two extracts are combined to obtain a tea extract.
[0015] Adjust the pH value of the tea extract to 5.0, and pass it through the cation exchange resin column with a flow rate of 5.0 ml / min. After loading the sample, rinse the cation exchange resin column with 500 ml of deionized water to remove impurities. After cleaning, the theanine is eluted with ammonia water with a concentration of 5% at a flow rate of 5 ml / min. After a certain period of time, the theanine was eluted completely by the ninhydrin color method. After the elution is complete, 1500ml of eluate is obtained. The eluate was evaporated to dryness in a water bath at a vacuum of 0.1 MPa and a temperature of 70°...
Example Embodiment
[0016] Example 2
[0017] Take 150g of Longjing tea powder, soak it in 1800ml deionized water at room temperature for 2h, then heat and boil for 1h, take the first extract, then add 1800ml deionized water to the tea residue, repeat the first extraction process , Take the second leaching solution. The two extracts are combined to obtain a tea extract.
[0018] Adjust the pH value of the tea extract to 5.0, and pass it through a cation exchange resin column with a flow rate of 7.0 ml / min. After loading the sample, rinse the cation exchange resin column with 500 ml of deionized water to remove impurities. After cleaning, the theanine was eluted with ammonia water with a volume concentration of 10% at a flow rate of 7 ml / min. After a certain period of time, the theanine was eluted completely by the ninhydrin color method. After the elution is complete, 600ml of eluate is obtained. The eluate was evaporated to dryness in a water bath at 70° C. at a vacuum of 0.1 MPa to obtain 12.1 g of...
Example Embodiment
[0019] Example 3
[0020] Take 150g of tea tree old leaves, soak them in 1800ml deionized water at room temperature for 2h, then heat and boil for 1h, take the first extract, then add 1800ml deionized water to the tea dregs, repeat the first extraction process , Take the second leaching solution. The two extracts are combined to obtain a tea extract.
[0021] Adjust the pH value of the tea extract to 5.5, and pass it through the cation exchange resin column with a flow rate of 3.0 ml / min. After loading the sample, rinse the cation exchange resin column with 500 ml of deionized water to remove impurities. After cleaning, the theanine is eluted with ammonia water with a volume concentration of 10% at a flow rate of 3.0 ml / min. After a certain period of time, the theanine was eluted completely by the ninhydrin color method. After the elution is complete, 1400ml of eluate is obtained. The eluate was put on a water bath at 70°C and evaporated to dryness under reduced pressure at a vacu...
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