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Microbiocidal formulation comprising essential oils or their derivatives

A technology for killing microbes and derivatives, which is applied in the direction of biocides, chemicals for biological control, biocides, etc., and can solve problems such as decay, instability, and lack of commercial availability of agricultural products

Inactive Publication Date: 2005-03-09
以色列国农业园林发展部农业研究组织(A.R.O.)富卡尼中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This damage has the potential to be severe and result in significant decay of the treated produce after a relatively short period of time
Another reason for their lack of commercial availability is their instability, as many essential oils are unstable and prone to breakdown before exerting their fungicidal activity

Method used

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  • Microbiocidal formulation comprising essential oils or their derivatives
  • Microbiocidal formulation comprising essential oils or their derivatives
  • Microbiocidal formulation comprising essential oils or their derivatives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Comparison of essential oils and imazalil in minimum inhibitory concentration and mode of action against Penicillium digitatum do not

[0087] The minimum inhibitory concentration (MIC) of more than 50 compounds found in the oil glands of citrus fruits was evaluated (in vitro) by determining the lowest concentration of the compound at which the pathogen would no longer grow (in vitro) in a petri dish. Table 5 shows the MICs of the more potent (compared to imazalil) compounds. In a sterile Petri dish (90 mm) containing 15 ml of molten Potato Dextrose Agar (PDA) (50 °C) was added the required amount of each test compound dissolved in 0.5 ml of acetone so that the final concentration was 1 mg / ml. Shake the dish gently to ensure even distribution of the test compound, then allow the agar to solidify. Discs (8 mm) of mycelium were excised from agar plate cultures of Penicillium digitatum that had not started to sporulate and were used to inoculate the Petri dishes. T...

Embodiment 2

[0119] Unblemished grapefruits are harvested from the orchard and sorted again the same day in the laboratory to remove damaged fruit. Rinse the grapefruit under running water and let it dry naturally in the air. Then use a tool to puncture three needles in three different parts of each fruit, and pierce the outer skin tissue to a depth of 1.5 mm to inoculate. Before each acupuncture, the tool was soaked in a spore suspension of Penicillium digitatum (10 6 spores / ml). The inoculated grapefruits were placed for 24 hours at a temperature of 17° C. and a relative humidity of 85%, then the grapefruits were divided into 6 groups, and the grapefruits of each group were processed by immersion in the following solutions for 2 minutes: (a) water; b) 25% ethanol (EtOH); (c) 25% ethanol with 0.2% geraniol; (d) 25% ethanol with 0.1% citral; (e) 25% ethanol with 0.2% citral Ethanol and (f) 25% ethanol with 0.5% citral dissolved.

[0120] The percentage of rotten fruit in each group was...

Embodiment 3

[0125] Lemons were inoculated according to the method shown in Example 2, and treated with 25% ethanol and without 25% ethanol to determine whether 5000 ppm emulsifier Tween 20 could stabilize the antifungal activity of citral. See results Figure 4 , which showed that Tween 20 at this concentration enhanced the activity of citral. In addition, the emulsifier avoids the phytotoxicity normally caused by citral when not dissolved in ethanol. It may be that this emulsifier enables the formation of stable emulsions in the form of microcolloids, avoiding phytotoxicity by homogeneously dispersing citral, which is itself phytotoxic at these concentrations. Thus, the combined use of Tween 20 and antioxidant BHA can replace ethanol in the formulation.

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Abstract

The present invention deals with a microbiocidal aqueous formulation comprising: (i) an effective amount of at least one essential oil component, or derivatives thereof, said derivatives thereof obtained by exposure to light or by oxidation, or mixtures thereof; and (ii) at least one additional stabilizer selected from the group consisting of ethanol in an amount of from 10% to about 50%, an emulsifier, an antioxidant, or an encapsulating agent. The invention is further directed to a method for inhibiting microbial development using said microbiocidal aqueous formulation.

Description

technical field [0001] The present invention relates to formulations and methods for inhibiting the growth of microorganisms. More specifically, the present invention relates to preparations and methods for inhibiting the growth of microorganisms in perishable agricultural products, household and human hygiene, etc., said preparations include essential oil components or derivatives thereof and stabilizers, said essential oil components Derivatives are obtained by exposing essential oil components to light or by oxidation. Background technique [0002] The rot of perishable agricultural products is caused by microbial infection. Usually, these agricultural products are stored for a sufficient period of time, and during the storage period conditions favorable to the multiplication of various microorganisms exist, so that it is very common that most of these agricultural products become infected. In addition to the obvious and substantial economic losses caused by this decay,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01N25/00A01N25/30A01N27/00A01N31/02A01N31/04A01N35/00A01N35/02A01N43/50A01N45/00A01N49/00A01N65/36A01P3/00A23B4/14A23B4/20A23B7/153A23L3/349A23L3/3544
CPCA01N45/00A01N65/36A01N27/00A01N49/00A01N31/02A01N65/00A01N35/02A01N25/10A01N25/30A01N43/50A01N25/02
Inventor 希姆雄·本-耶霍舒亚
Owner 以色列国农业园林发展部农业研究组织(A.R.O.)富卡尼中心
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