Method for refining long-chain biatomic acid

Abstract

The invention relates to a kind of method for abstracting and refining long chain binary acid by water phase from C10-C18 regular normal-structure alkane or mixed normal-structure alkane fermenting liquor. The invention solves the disadvantages of low purity and high chroma in the production made by existing water phase. The method can decline the content of impurity such as protein and chroma effectively, and can be combined with the steps of thalline separation, acid-out and crystallization, drying and so on, to manufacture long chain binary acid product with total acid content more than 99wt% and white appearance. It uses the technique combining long chain binary mono-salt crystallization and salting-out, so it can leave the step of binary acid recovering treatment with salting-out mother liquor and mono-salt filter liquor out, save treatment cost, and improve product yield.

Description

technical field

[0001] The invention relates to a method for extracting and refining long-chain dibasic acids from fermentation broth, especially from one or more C 10 ~C 18 A method for extracting and refining long-chain dibasic acids from n-alkane fermentation broth. Background technique

[0002] Long-chain dibasic acids are metabolites obtained from the fermentation of normal alkanes by microorganisms. Its fermentation broth is a complex multiphase system, which contains unreacted normal alkanes, microbial cells and debris, unused medium and metabolites, and microbial secretions, etc., especially containing a large amount of impurities such as proteins and pigments , affecting the purity and appearance quality of the product.

[0003] At present, the methods for refining long-chain dibasic acids are generally divided into two categories: solvent method and aqueous method. Although the solvent method can solve the above problems, the use of the method is greatly restri...