Reagent box for detecting content of protein carbonyl group

Abstract

A reagent kit for detecting carbonyl content of protein in tissue organ, serum and cell consists of a set of seven bottles reagent used coordinately and used as a complete set in detection. Each bottle is packed with reagent in set concentration and set volume as bottle one is placed in foam box with ice bag and the others are placed in the same box made by hard paper-plastic material.

Description

Technical field [0001] The invention relates to a kit for detecting the carbonyl content of protein, which is suitable for detecting the degree of protein oxidation damage in various tissues, organs, serum, cultured cells and organelles. Background technique [0002] In 1987, Oliver et al proposed that protein carbonyl content could be used as an index to determine protein oxidative damage. Over the years, domestic and foreign countries have been working on effective detection methods for protein carbonyl content to evaluate the degree of protein oxidative damage. Methods include sodium borohydride method, fluorescein hydrazine method, fluorescamine method, immunoblotting method, etc. These methods either require expensive equipment or expensive reagents, and the sample processing is very complicated. The 2,4-dinitrophenylhydrazine (DNPH) colorimetric method is a classic method for the determination of protein carbonyl content. It is simple and effective in detecting protein...

AI Technical Summary

Problems solved by technology

[0002] In 1987, Oliver et al proposed that protein carbonyl content could be used as an index to judge protein oxidative damage. Over the years, domestic and foreign countries have been working on effective detection methods of protein carbonyl content to evaluate the degree of protein oxidative damage. Methods include sodium borohydride method, fluorescein hydrazine method, fluorescein amine method, immunohybridization method, etc. These methods either require expensive equipment or expensive reagents, and the sample processing is very complicated

The 2,4-dinitrophenylhydrazine (DNPH) colorimetric method is a classic method for determining protein carbonyl content. It is simple and effective in detecting protein carbonyl content. It is inconvenient, and there will be large systematic errors due to the accuracy of the experimenters and instruments, and some scholars at home and abroad have many deficiencies in designing experiments, which will affect the final experimental results

There is no domestic kit for detecting protein oxidative damage, and some foreign companies sell specific antibodies, but they are relatively expensive

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