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Monacrosporium elegans bactericidal agent with nematode killing function and its preparation method and use thereof

A technology for killing nematodes and inoculants, which is applied to Acremonium monosporum inoculants and their preparation methods and application fields, and can solve problems such as environmental pollution, drug resistance, and harm to human health.

Inactive Publication Date: 2005-09-21
YUNNAN UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

However, with the advancement of science and technology, it has been found that many chemical nematicides have side effects, which not only produce drug resistance, but also cause environmental pollution and endanger human health. Many of them have been banned or will be banned one after another.

Method used

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  • Monacrosporium elegans bactericidal agent with nematode killing function and its preparation method and use thereof

Examples

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Embodiment Construction

[0040] The following are examples of the present invention, but the content of the present invention is not limited thereto.

[0041] The YMF1.00027 strain was inoculated on the modified PDA slant, and the medium formula was: 1% glucose; 100g potato boiled juice, 1.8% agar; the pH was natural. The YMF1.00024 mycelium was inoculated on the culture medium and cultured at 22°C for 8 days to obtain test tube species.

[0042] The test tube seed is inoculated in the 250ml Erlenmeyer flask liquid medium (60ml of every bottle), and the liquid medium formula is: 0.1% glucose, 0.1% ammonium sulfate, 0.05% magnesium sulfate, 100g potatoes are boiled into juice, 0.001% ferrous sulfate, Make up volume to 1 L with water, pH 6.5. Each 250ml Erlenmeyer flask was divided into 60ml of culture solution, and a small amount of nematode was added as an inducer, sterilized at 121°C for 20 minutes, inserted into the bacterial block, and cultured on a shaker (200rpm) at 26°C for 6 days.

[0043] Ad...

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Abstract

This invention relates to a Monacrosporium elegans bacteriostatic and its process and application, belonging to microbial pesticide technology region, with the preparing bacterial strain being Monacrosporium elegans, YMF1.00027, adopting normal process to prepare. Wherein, liquid fermenting culture medium dispense includes: 0.1% of glucose, 0.1% of ammonia sulfate, 0.05% of addex-magnesium, and liquor boiled from 100g potatoes, 0.001% of copperas, and filling waters to complement volume to 1L, PH6.5. The method for extracting effective composition to kill nemathelminth from liquid fermenting production is characterized by filtering fermented culture and dewatering supernatant with ammonia sulfate. The bacteriostatic is effective for killing Panagrellus redivivus and pine beam nemathelminths, and has broad aspect of application.

Description

Technical field: [0001] The invention relates to a Caenorhamona elegans fungus agent with a nematicide function, a preparation method and application thereof, and belongs to the technical field of biological pesticides. Background technique: [0002] Plant nematodes are a common plant disease worldwide. There are more than 70 known species of root-knot nematodes alone, which harm more than 3,000 plants and cause huge losses every year. According to the statistics of the Food and Agriculture Organization of the United Nations, the global losses caused by nematodes are as high as 100 billion U.S. dollars every year. In 2001, the losses caused by nematode damage to crops in my country were as high as 23.3 billion yuan. Pine xylophilus is known as smokeless forest fires, which have seriously occurred in some areas such as Jiangsu, Zhejiang, Guangdong, Shandong, Anhui, Hubei, Shanghai, Taiwan, Hong Kong, etc., and have a tendency to spread to the whole country. What's more serio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04C12N1/14
Inventor 杨金奎张克勤周薇
Owner YUNNAN UNIV
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