Monacrosporium elegans bactericidal agent with nematode killing function and its preparation method and use thereof

Abstract

The invention relates to a Caenorhamona elegans fungus agent with the function of killing nematodes, a preparation method and application thereof, and belongs to the technical field of microbial pesticides. The production strain of this bacterial agent is Monacrosporium elegans (Monacrosporium elegans), YMF1.00027, prepared by conventional methods, wherein the liquid fermentation medium formula is: 0.1% glucose, 0.1% ammonium sulfate, 0.05% magnesium sulfate, 100g potatoes boiled Juice, 0.001% ferrous sulfate, make up volume to 1 L with water, pH 6.5. The method for extracting the nematicidal active ingredient from the liquid fermentation product is that the fermentation culture is filtered, and the supernatant is concentrated with ammonium sulfate to be used as a nematicidal medicinal liquid. The bacterial agent of the invention has high toxicity to Panagrellus redivivus nematodes and pine wood nematodes, and has good application prospects.

Description

Technical field: [0001] The invention relates to a Caenorhamona elegans fungus agent with a nematicide function, a preparation method and application thereof, and belongs to the technical field of biological pesticides. Background technique: [0002] Plant nematodes are a common plant disease worldwide. There are more than 70 known species of root-knot nematodes alone, which harm more than 3,000 plants and cause huge losses every year. According to the statistics of the Food and Agriculture Organization of the United Nations, the global losses caused by nematodes are as high as 100 billion U.S. dollars every year. In 2001, the losses caused by nematode damage to crops in my country were as high as 23.3 billion yuan. Pine xylophilus is known as smokeless forest fires, which have seriously occurred in some areas such as Jiangsu, Zhejiang, Guangdong, Shandong, Anhui, Hubei, Shanghai, Taiwan, Hong Kong, etc., and have a tendency to spread to the whole country. What's more serio...

AI Technical Summary

Problems solved by technology

However, with the advancement of science and technology, it has been found that many chemical nematicides have side effects, which not only produce drug resistance, but also cause environmental pollution and endanger human health. Many of them have been banned or will be banned one after another.

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