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Bielement beacon of wavelength conversion

A wavelength conversion, molecular beacon technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, luminescent materials, etc., can solve the problem that the fluorescence intensity of fluorescent molecules cannot reach the maximum, and cannot be convenient and fast problem, to achieve high specificity and high sensitivity

Inactive Publication Date: 2005-11-16
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When multiple molecular beacons carrying different fluorescent molecules are used for simultaneous detection, a variety of different excitation light sources are required, which makes it difficult to achieve convenient and fast detection of multiple target molecules at the same time, and due to the limitations of multiple excitation light sources characteristics, resulting in the fluorescence intensity of fluorescent molecules cannot reach the maximum

Method used

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  • Bielement beacon of wavelength conversion
  • Bielement beacon of wavelength conversion

Examples

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Effect test

Embodiment 1

[0014] Example 1: wavelength conversion bimolecular beacons, when detecting two target molecules 3 of a cell, the beacon donor 1 in the two pairs of molecular beacons uses fluorescein, and the beacon receptors in the two pairs of molecular beacons Body 2 uses Texas Red and FITC.

Embodiment 2

[0015] Example 2: wavelength conversion bimolecular beacons, when detecting two target molecules 3 of a cell, the beacon donor 1 in the two pairs of molecular beacons uses coumarin, and the beacons in the two pairs of molecular beacons Receptor 2 uses Fluorescent Yellow and EADNS.

Embodiment 3

[0016] Example 3: wavelength conversion bimolecular beacons, when detecting two target molecules 3 of a cell, the beacon donor 1 in the two pairs of molecular beacons uses Texas Red, and the beacons in the two pairs of molecular beacons receive Body 2 uses fluorescent yellow and EADNS.

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Abstract

A double molecule beacon of wavelength conversion consists of beacon donator and beacon acceptor as the beacon donator in two pairs of molecule beacon applies the same fluorescent molecule and the beacon acceptor in two pairs of molecule beacon applies dezhou red and FITC, fluorescent yellow and EADNS or tetramethyl luodan bright and Cy3. The fluorescent molecule of beacon acceptor is different from fluorescent molecule of beacon donator.

Description

technical field [0001] The invention relates to the development of molecular biological products and early diagnosis reagents for tumors, in particular to a wavelength-converted bimolecular beacon. Background technique [0002] The molecular beacon (Molecule Beacon, MBs) designed according to the phenomenon of fluorescence resonance energy transfer (FRET) is a new molecular biology method with strong sensitivity, high specificity, and extremely simple and convenient operation (Mitchell, P.Turning the spotlight on cellular imaging. Nat. Biotechmol., 2001, 19, 1013-1017.). In recent years, the research on molecular beacons has developed rapidly, and has been widely used in real-time monitoring of polymerase chain reaction, rapid analysis of gene mutations, detection of DNA / RNA, kinetics of DNA / RNA hybridization, and DNA / protein interaction Research, etc., and its application fields are still expanding (Tyagi S, Bratu DP, Kramer FR. Multicolormolecular beacons for allele discr...

Claims

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Application Information

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IPC IPC(8): G01N21/76
Inventor 贺大林赵军何辉
Owner XI AN JIAOTONG UNIV
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