Making method of body fluid cell lump paraffin section

A technology for paraffin sections and body fluid cells, applied in the preparation of test samples, instruments, teaching models, etc., can solve the problems of inability to check immunohistochemical staining, inability to accurately detect tumor patients, inability to detect cell molecular markers, etc., to achieve Accurate treatment guidance, improved diagnostic accuracy, clear background effects

Inactive Publication Date: 2006-02-22
徐华
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Problems solved by technology

[0002] Under the action of carcinogenic factors, the nature of human cells will gradually change qualitatively and become cancer cells. Under the influence of long-term carcinogenic factors, the tumor cells formed by the cells will develop into malignant tumors when the number of tumor cells increases to a certain number. , in order to diagnose the disease, prescribe the right medicine, and strive for the best treatment, time and effect, one of the methods currently used by the medical department for patients is to find cancer cells in body fluids, and generally use a centrifuge to make a centrifugal sedimentation

Method used

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Embodiment Construction

[0015] Compared with the results of ordinary centrifugal smears in the detection of 50 cases of pleural effusion, the present invention found 5 cases of cancer cells, and there was no significant difference in the detection of tumor cells, but the present invention obtained more tumor cells, and immunohistochemical staining could be performed , the immunohistochemical staining has a clear background and good color development, especially for the detection of drug-resistant gene expression products, which is of great help to tumor chemotherapy, and can be used to classify tumor cells through immunohistochemical staining to improve the quality of diagnosis and perform cell For marker detection, it is sometimes difficult to distinguish degenerated mesothelial cells from degenerated tumor cells. Using immunohistochemical staining to mark cell types can improve the accuracy of diagnosis, classify tumors and provide more accurate treatment guidance sexual opinion.

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Abstract

This invention relates to body liquid cell olefin slice making process, which comprises pre-processing, decenter deposition and fixing dehydrate wax impregnation, burying, slicing and HE dying and sealing. The invention works together with daily work with clear background of HE and immune organization dying without dark background of agar burying and processing the molecule burying and improves the accuracy of the body liquid cell diagnose.

Description

technical field [0001] The invention is one of the detection methods for clinical medical detection of tumor cells and cell molecular markers. Background technique [0002] Under the action of carcinogenic factors, the nature of human cells will gradually change qualitatively and become cancer cells. Under the influence of long-term carcinogenic factors, the tumor cells formed by the cells will develop into malignant tumors when the number of tumor cells increases to a certain number. , in order to diagnose the disease, prescribe the right medicine, and strive for the best treatment, time and effect, one of the methods currently used by the medical department for patients is to find cancer cells in body fluids, and generally use a centrifuge to make a centrifugal sedimentation smear, or use agar It is simple and quick to use a smear centrifuge to embed cell block paraffin sections, but the equipment is more expensive, and cannot be used for immunohistochemical staining, cell...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/34G01N1/30G09B23/28
Inventor 徐华
Owner 徐华
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