New use of natural abscisic acid i preparing tumour cell

A technology of natural abscisic acid and differentiation inducer, applied in the field of natural substances, can solve the problems of limited development and application, toxic and side effects, and achieve the effects of inhibiting the growth of cancer cells and tumors, with less toxic and side effects, and simple operation.

Inactive Publication Date: 2006-03-22
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As we all know, retinoids are the most effective and deeply researched differentiation inducers discov

Method used

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  • New use of natural abscisic acid i preparing tumour cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Study on the differentiation induction of prostate cancer cells by natural abscisic acid:

[0018] The prostate cancer DU-145 cells in the logarithmic growth phase were divided into two groups, the experimental group and the control group, and the experimental group was added with 20ul2×10 -2 g / L ABA (dissolved in absolute ethanol), make the final concentration of ABA in the nutrient solution be 10 -2 g / L, the control group was added with 20ul of absolute ethanol, the concentration of calf serum in the culture solution was 130mI / L, and the cells were cultured by conventional methods. Routinely change culture medium after adding medicine 48h, maintain identical drug concentration (ABA concentration is 10 -2 g / L). During the culture process, the morphological changes of DU-145 cells were dynamically observed with an inverted optical microscope.

[0019] result:

[0020] Observation of cell morphology and ultrastructure: DU-145 cells cultured and treated wit...

Embodiment 2

[0023] Example 2: Study on the differentiation induction of promyelocytic leukemia HL-60 cells by natural abscisic acid:

[0024] The promyelocytic leukemia HL-60 cells in the logarithmic growth phase were divided into the experimental group and the control group, and the experimental group was added with 10ul 5×10 -1 g / L ABA (dissolved in absolute ethanol), so that the final concentration of ABA in the culture medium was 1×10 -1 g / L, the control group was added with 10ul of absolute ethanol, the concentration of calf serum in the culture solution was 130mI / L, and the cells were cultured by conventional methods. After adding the drug for 48 hours, the culture medium was routinely replaced to maintain the same drug concentration (the concentration of ABA was 1×10 -1 g / L). During the culture process, the morphological changes of HL-60 cells were dynamically observed by transmission electron microscope.

[0025] Observation of cell morphology and ultrastructure: HL-60 cells cu...

Embodiment 3

[0030]3. Effect of natural abscisic acid on the ability of xenograft tumor formation in nude mice

[0031] 1).Material

[0032] (1) 3-week-old Balb / c.nu nude mice, with an average body weight of about 20 g, are all male.

[0033] (2) Barrier system for SPF rearing of nude mice.

[0034] (3) Human oral cancer Tca8113 cell line 2.

[0035] 2) method

[0036] 2.1) Preparation of inoculated cells

[0037] Cultivate Tca8113 cells to the logarithmic growth phase according to the method of test 1, detach and disperse the adherent tumor cells with digestive solution, wash them twice with serum-free culture medium, count the viable cells, and adjust to 2 with PBS solution. ×10 7 / ml cell suspension. Use a syringe with a No. 6 needle to draw an appropriate amount of tumor cell suspension to inoculate nude mice. Each animal is inoculated to one site (inoculated in the armpit of nude mice), and each site is injected with 0.2ml of tumor cell suspension, containing 4 live cells. ×10 ...

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Abstract

The present invention relates to new use of natural medicine, and is the new use of natural abscisic acid in tumor treating medicine, and especially the new use of natural abscisic acid in preparing tumor cell differentiation inducing agent. Natural abscisic acid in the concentration of 1 microgram/L to g/L can inhibit the proliferation of tumor cell and induce the transformation of tumor cell to normal cell effectively. The present invention has the advantages of simple operation, high effect, less toxic side effect, etc.

Description

[0001] 1. Field: [0002] The invention relates to a new application of natural substances, in particular to a new application of natural abscisic acid. 2. Background technology: [0003] For a long time, people have been fighting unremittingly against tumors, one of the main diseases that threaten people's health. Surgery, radiotherapy, and chemotherapy have become the three major means of tumor treatment. However, these three methods have large toxic and side effects and poor selectivity. In recent years, tumor differentiation induction and differentiation therapy have been widely carried out around the world. Four symposiums have been held in the world, and the differentiation induction therapy of tumor cells has been pushed from basic to clinical, thus opening up a new way for tumor treatment . [0004] A large number of studies have confirmed that the application of some chemical substances can reverse malignant tumor cells and differentiate into normal cells; these sub...

Claims

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Application Information

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IPC IPC(8): A61K31/19A61K9/00A61P35/00
Inventor 谭红雷宝良李志东周金燕杨杰钟娟
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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