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Method for extracting abalone polysaccharide

A technique for extracting polysaccharides from abalone, which is applied in the field of biological products, can solve the problems of extracting less polysaccharides and rare ones, and achieve the effects of saving usage, making full use of resources, and reasonable processes

Active Publication Date: 2006-03-22
DALIAN POLYTECHNIC UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on extracting polysaccharides from animals, and even rarer studies on extracting polysaccharides from marine animals
There is no report on the effective extraction of abalone polysaccharides from abalone

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0049] Take 1,000 grams of abalone as a whole, add 5,000 grams of water, homogenize it with a tissue masher, add 25,000 grams of water, stir evenly, extract at a temperature of 50°C for 5 hours, and centrifuge at a high speed to obtain a supernatant and a precipitate. Add 25,000 grams of water to the precipitate, stir evenly, extract at a temperature of 80°C for 3 hours, and centrifuge at a high speed to obtain supernatant and precipitate. The resulting supernatants were pooled. Concentrate the supernatant in vacuum until the polysaccharide content is 3% (detected by the phenol-sulfuric acid method); add 21,000 ml of 95% ethanol 3 times the volume to the concentrated solution, alcohol precipitation for 16 hours at a temperature of 4°C, and high-speed centrifugation for 10 minutes Left and right, centrifuge insoluble matter was obtained. The insoluble matter was vacuum-dried at a temperature of 60° C. to obtain 61.6 grams of dried abalone crude polysaccharide. Use a pulverize...

example 2

[0051] Take 1,000 grams of freeze-dried whole abalone powder, add 50,000 grams of water, stir well, add 0.1N sodium hydroxide solution to it, adjust the pH to 9, and extract it at a temperature of 20°C for 6 hours, then use 6mol / L HCl Neutralize to pH 7, centrifuge at high speed to obtain supernatant and precipitate. Add 30,000 grams of water to the precipitate, stir evenly, add 0.5 mol / L NaOH solution to it, adjust the pH to 8, and after leaching for 3 hours at a temperature of 80°C, neutralize it with 6 mol / L HCl until the pH is 7. Centrifuge at high speed to obtain supernatant and precipitate. The resulting supernatants were pooled. Concentration, alcoholization, centrifugation as example 1. The insoluble matter was vacuum-dried at a temperature of 50° C. to obtain 298 grams of dried abalone crude polysaccharide. Use a pulverizer to pulverize to below 160 mesh. Its polysaccharide content is 9.3% as detected by the phenol-sulfuric acid method.

example 3

[0053] Get 1000 grams of abalone gastropods, process as example 1, use 20KHZ ultrasonic treatment for 60 minutes, high-speed centrifugation, obtain centrifugal supernatant and sediment. The precipitate was added to 25,000 grams of water, ultrasonically treated at 27KHZ for 20 minutes, and centrifuged at a high speed to obtain the centrifuged supernatant and the precipitate. The resulting supernatants were pooled. Concentration, alcoholization, centrifugation as example 1. The insoluble matter was vacuum-dried at a temperature of 50° C. to obtain 65.2 grams of dried abalone polysaccharide. Use a pulverizer to pulverize to below 160 mesh. Its polysaccharide content is 8.8%.

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Abstract

The extraction process of abalone polysaccharide includes the following steps: taking abalone meat, breaking wall in tissue stamping machine, mixing with water homogeneously, lixiviating at 20-80 deg.c for 2-6 hr, centrifugally separating, re-extracting the insoluble matter and merging supernatant; concentrating the extracted liquid to polysaccharide content of 1.5-3.0 5, alcohol precipitating at 0-4 deg.c for 12-16 hr, and vacuum drying or spray drying to obtain the product. During the extraction, alkali, ultrasonic vibration and / or enzyme, single or compound, may be added. The present invention creates the technological path of extracting abalone polysaccharide and provides technological foundation for the research of medicinal use of abalone polysaccharide.

Description

technical field [0001] The invention relates to a polysaccharide extraction method, which belongs to the field of biological products. Background technique [0002] In recent years, with the development of molecular biology, people have gradually realized that polysaccharides, proteins, and nucleic acids are one of the three types of biological macromolecules involved in the essence of life activities. Polysaccharides are a class of biomacromolecular substances with a wide range of biological activities, also known as polysaccharides. They are not only essential components for human life, but also exist in all cell membrane structures and participate in various life functions. In recent years, it has been found that polysaccharides can enhance the immune function of the human body, and also have physiological activities such as anti-cancer, anti-radiation, anti-inflammation, and hypoglycemic, so it has received widespread attention and attention. [0003] At present, most o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003A61P35/00
Inventor 朱蓓薇李冬梅殷红玲董秀萍宋玉李铖铖姜丹
Owner DALIAN POLYTECHNIC UNIVERSITY
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