Artificial induction method for a mushroom (Tricholoma matsutake(S. Ito et Imai)Sing)

Abstract

The invention relates to a method for the inducing culture of mushroom in the artificial bacterium pool, which comprises: a) washing the container with washing agent, cleaning it up and drying; b) spraying the container and the container cover with 70% alcohol and 0.05-1.0% sodium hypochlorite solvent for disinfection on the operating platform which is surface sterilized by 70% alcohol, reserving the antiseptic solution for 5-20 minutes, the antiseptic solution consumption is 5-20 ml for 100 ml container; c) putting the container with the mouth downfacing on the operating platform with surface sterilization, drying in the open for 30-40 minutes; d) putting the culture soil which is mixed with absolute ethyl alcohol (the volume percentage is 1.4%) and olive oil (with the concentration of the oil is 0.5-2.0%) and sterilized in high pressure to the container directly, inoculating the mushroom bacterium block or solvent, covering or sealing it, proceeding inducing culture in dark condition with the temperature being 20-30 deg.

Description

technical field [0001] The invention relates to a method for inducing artificial bacteria ponds of matsutake mushrooms, in particular to an experimental method suitable for non-laboratory operations. Background technique [0002] Tricholoma matsutake (S.Ito.et.Imai) Sing.] - one of the most valuable mycorrhizal edible fungi in the world, is a national second-class protected plant. In my country, it is mainly distributed in Jilin and Heilongjiang in the northeast, Yunnan and Sichuan in the southwest, and sporadically distributed in other provinces such as Shanxi, Anhui, Taiwan, and Tibet. Foreign countries are mainly distributed in Japan, North Korea, Russia and other countries. Matsutake mushroom is a delicious food rich in protein, multivitamins and a large amount of sugar. In the world, Japanese people like to eat Matsutake mushroom. In recent years, due to the deterioration of the environment and the expansion of demand, Japan's local production has been far from enough ...

AI Technical Summary

Problems solved by technology

However, there are certain defects in these induction methods—for example, Yamada (1999b) and Guerin (2003) all used autoclaving to sterilize the induction containers, because large-capacity plastic containers or relatively cheap plastic utensils Most of them are not resistant to high temperature. Sterilization with gamma-rays is a solution to the problem (Tomo Teramura, 2000), but the cost is high, and it is impossible for general laboratories or experimental stations to have such facilities

Therefore, although these induction methods are theoretically feasible, they are very difficult in practice.