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Creatinine content determination and creatinine diagnostic reagent kit

A diagnostic kit and measurement method technology, applied in the field of medical testing and measurement, can solve problems such as slowness, reduced accuracy of reagent testing, and inconvenient testing

Inactive Publication Date: 2006-05-31
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One of its disadvantages is that while the system generates the reduced nicotinamide coenzyme required for the reaction, it also offsets some of the enzymes such as alanine aminotransferase, aspartate aminotransferase, urea, ammonia, creatinine, and carbon dioxide. activity, causing the accuracy of the reagent test to be greatly reduced
The second disadvantage is that the reagent must be reacted for a period of time before the formal test in order to produce enough reduced nicotinamide coenzyme, which results in slow results and brings great inconvenience to the test

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment one (single agent)

[0081] The creatinine diagnostic kit of the present embodiment comprises:

[0082] Buffer 80mmol / l

[0083] Oxidized coenzyme 1mmol / l

[0084] Ethanol 3mmol / l

[0085] Creatinase 8000U / l

[0086] Creatinase 8000U / l

[0087] Sarcosine oxidase 6000U / l

[0088] Aldehyde dehydrogenase 8000U / l

[0089] Catalase 5000U / l

[0090] Stabilizer 50% (total volume)

[0091] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the creatinine sample to the reagent to be tested is 1 / 25, and the reaction direction is positive reaction.

[0092] After the sample and reagents are added and allowed to mix, the following reactions occur:

[0093] Creatinine + water Creatinase Creatine

[0094] Creatine + water creatinase Sarcosine + urea

[0095] Sarcosine + oxygen + water Sarcosine oxidase Hydrogen peroxide + forma...

Embodiment 2

[0103] Embodiment two (two doses)

[0104] The creatinine diagnostic reagent of the present embodiment has:

[0105] Reagent I

[0106] Buffer 100mmol / l

[0107] Oxidized coenzyme 3mmol / l

[0108] Ethanol 8mmol / l

[0109] Creatinase 14000U / l

[0110] Sarcosine oxidase 8000U / l

[0111]Aldehyde dehydrogenase 14000U / l

[0112] Catalase 8000U / l

[0113] Stabilizer 50% (total volume)

[0114] Reagent II

[0115] Buffer 100mmol / l

[0116] Creatinase 14000U / l

[0117] Stabilizer 50% (total volume)

[0118] When measuring creatinine content, the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 340nm, the secondary wavelength of the test is above 405nm, the volume ratio of the creatinine sample to the reagent is 1 / 25, and the reaction direction is positive reaction.

[0119] The specific measurement steps are:

[0120] Creatinine + water Creatinase Creatine

[0121] Creatine + water creatinase Sarcosine + ...

Embodiment 3

[0130] Embodiment three (three doses)

[0131] The creatinine diagnostic reagent of the present embodiment is three doses, has:

[0132] Reagent I

[0133] Buffer 120mmol / l

[0134] Oxidized coenzyme 5mmol / l

[0135] Ethanol 12mmol / l

[0136] Stabilizer 20mmol / l

[0137] Reagent II

[0138] Buffer 120mmol / l

[0139] Creatinase 20000U / l

[0140] Sarcosine oxidase 10000U / l

[0141] Aldehyde dehydrogenase 20000U / l

[0142] Catalase 10000U / l

[0143] Stabilizer 50% (total volume)

[0144] Reagent III

[0145] Buffer 120mmol / l

[0146] Creatinase 20000U / l

[0147] Stabilizer 50% (total volume)

[0148] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the creatinine sample to be tested is 1 / 25, and the reaction direction is positive reaction.

[0149] The specific measurement steps are:

[0150] Creatinine + water Creatinase Creatine

[0151] ...

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PUM

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Abstract

The invention is about a method of measuring the content of creatinine, and it also concerns the reagent box of creatinine diagnosis. This invention belongs to the field of medical testing and measuring technology. The reagent box is consisted of buffer solution, oxidized coenzyme, alcohol, creatininase, creatinase, sarcosine onidase, aldehyde dehydrogenase and stabilizer. Firstly, we cause an enzyme-coupled reaction through mixing the sample and the reagent according to a certain proportion of volume; secondly, put the final reactant under the biochemical analyzer and test the absorbance variational situation (speed) of dominant wavelength; then we can get the content of creatinine. By using this invention, we can get the necessary measuring result with high sensitiveness and fine precision through biochemical analyzer, and the result would not be contaminated by material of internal and exogenous sources. Thus, this method can be conveniently promoted and applied.

Description

technical field [0001] The invention relates to a method for measuring creatinine content, and at the same time, the invention also relates to a creatinine diagnostic kit for realizing the method, which belongs to the technical field of medical examination and measurement. Background technique [0002] Determination of creatinine mainly includes chemical determination method (Jaffe method), enzymatic method, high performance liquid chromatography and capillary electrophoresis. [0003] Chemical determination method - low cost, easy to operate, is currently one of the most commonly used methods for the determination of creatinine in China. The creatinine in the specimen interacts with picrate to form a yellow-red complex of picric acid and creatinine. The disadvantage of this method is that the specificity is not high, because vitamin C, acetone, acetoacetic acid, methyldopa and high concentration of glucose, protein and some antibiotics such as penicillin G, cefoxitin, cefa...

Claims

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Application Information

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IPC IPC(8): C12Q1/32
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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